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Descripción
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| Winter dormancy is the strategy used by perennial plants to survive the winter harsh conditions in temperate and cold regions. Relation between changes in epigenetic marks pattern throughout different stages of winter dormancy of woody plants is barely-known. Among the important epigenetic marks, 5-methyl cytosine (5mC) regulates gene expression in animals and plants. DNA demethylation is performed in Arabidopsis by specific 5mC DNA glycosylases of the DEMETER/ROS1 family. We have identified in poplar two homologs to the Arabidopsis DME (PtaDML8 and PtaDML10) that are over-represented during the growing season. PtaDML8/PtaDML10 knock-down (KD plants show a delay in resuming growth after dormancy. The goals of this stay were: a) to quantify global DNA methylation changes by HPLC, weekly, during winter dormancy in the apical meristem of poplar trees from January (deep dormant stay) to the time point of bud burst at the beginning of the spring; b) to quantify global DNA methylation changes by HPLC of PtaDML8/PtaDML10 KD poplar trees vs. the wild type (WT); c) to identify the locus/i affected by the demethylation of PtaDML8/PtaDML10 by whole-genome bisulfite sequencing and to learn how to use bioinformatics tools to analyze the data obtained after the application of this technique with DNA samples of WT in comparison to PtaDML8/PtaDML10 KD lines | |
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Internacional
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Si |
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Lugar
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Orleans, Francia |
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Tipo
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Fecha inicio
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25/05/2015 |
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Fecha fin
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24/08/2015 |