Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Is Microarray Analysis Really Useful and Sufficient to Diagnose Nut Allergy in the Mediterranean Area?
Year:2015
Research Areas
  • Natural sciences and health sciences,
  • Earth sciences,
  • Molecular, cellular and genetic biology,
  • Biological sciences,
  • Biology,
  • Ethics in natural sciences,
  • Environmental biology,
  • Plant genetics
Information
Abstract
Background: Component-based diagnosis on multiplex platforms is widely used in food allergy but its clinical performance has not been evaluated in nut allergy. Objective: To assess the diagnostic performance of a commercial protein microarray in the determination of specific IgE (sIgE) in peanut, hazelnut, and walnut allergy. Methods: sIgE was measured in 36 peanut-allergic, 36 hazelnut-allergic, and 44 walnut-allergic patients by ISAC 112, and subsequently, sIgE against available components was determined by ImmunoCAP in patients with negative ISAC results. ImmunoCAP was also used to measure sIgE to Ara h 9, Cor a 8, and Jug r 3 in a subgroup of lipid transfer protein (LTP)-sensitized nut-allergic patients (positive skin prick test to LTP-enriched extract). sIgE levels by ImmunoCAP were compared with ISAC ranges. Results: Most peanut-, hazelnut-, and walnut-allergic patients were sensitized to the corresponding nut LTP (Ara h 9, 66.7%; Cor a 8, 80.5%; Jug r 3, 84% respectively). However, ISAC did not detect sIgE in 33.3% of peanut-allergic patients, 13.9% of hazelnut-allergic patients, or 13.6% of walnut-allergic patients. sIgE determination by ImmunoCAP detected sensitization to Ara h 9, Cor a 8, and Jug r 3 in, respectively, 61.5% of peanut-allergic patients, 60% of hazelnut-allergic patients, and 88.3% of walnut-allergic patients with negative ISAC results. In the subgroup of peach LTP?sensitized patients, Ara h 9 sIgE was detected in more cases by ImmunoCAP than by ISAC (94.4% vs 72.2%, P<.05). Similar rates of Cor a 8 and Jug r 3 sensitization were detected by both techniques. Conclusions: The diagnostic performance of ISAC was adequate for hazelnut and walnut allergy but not for peanut allergy. sIgE sensitivity against Ara h 9 in ISAC needs to be improved.
International
Si
JCR
Si
Title
Journal of Investigational Allergology And Clinical Immunology
ISBN
1018-9068
Impact factor JCR
2,642
Impact info
Volume
26
Journal number
From page
31
To page
39
Month
SIN MES
Ranking
Participants
  • Autor: MJ Goikoetxea (Department of Allergy and Clinical Immunology, Clinica Universidad de Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain)
  • Autor: CM D¿Amelio (Department of Allergy and Clinical Immunology, Clinica Universidad de Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain)
  • Autor: R Martínez-Aranguren (Department of Allergy and Clinical Immunology, Clinica Universidad de Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain)
  • Autor: P Gamboa (Allergy Department, Hospital de Basurto, Bilbao, Spain)
  • Autor: BE García (Allergy Department, Complejo Hospitalario de Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain)
  • Autor: F Gómez (Allergy Department, Hospital Regional Universitario de Málaga, IBIMA, Málaga, Spain)
  • Autor: J Fernández (Allergy Department, Hospital General Universitario de Alicante, Alicante, Spain)
  • Autor: J Bartra (Allergy, Respiratory, and Respiratory Allergy Department, Hospital Clinic, Universitat de Barcelona, Institut d¿Investigacions Biomédiques August Pi i Sunyer)
  • Autor: A Parra (Allergy Department, Hospital de A Coruña, A Coruña, Spain)
  • Autor: MI Alvarado (Allergy Department, Hospital Virgen del Puerto, Plasencia, Cáceres, Spain)
  • Autor: MI Alonso (Allergy Department, Hospital de Alcorcón, Madrid, Spain)
  • Autor: E González (Allergy Department, Hospital de Fuenlabrada, Madrid, Spain)
  • Autor: S Terrados (Allergy Department, Hospital Ramón y Cajal, Madrid, Spain)
  • Autor: C Moya (Allergy Department, Complejo Hopitalario Torrecárdenas, Almeria, Spain)
  • Autor: N Blanca-López (Allergy Department, Hospital Infanta Leonor, Madrid, Spain)
  • Autor: F Feo-Brito (Allergy Department, Hospital General Universitario de Ciudad Real, Ciudad Real, Spain)
  • Autor: M Villalba (Molecular Biology Department, Facultad de Químicas, Universidad Complutense, Madrid, Spain)
  • Autor: Araceli Diaz Perales (UPM)
  • Autor: ML Sanz (Department of Allergy and Clinical Immunology, Clinica Universidad de Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Biotecnología Vegetal
S2i 2019 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)