Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
A Recombinant Sal k 1 Isoform as an Alternative to the Polymorphic Allergen from Salsola kali Pollen for Allergy Diagnosis
Year:2015
Research Areas
  • Natural sciences and health sciences,
  • Molecular, cellular and genetic biology,
  • Biological sciences,
  • Biology,
  • Biological engineering,
  • Plants and animal biology and ecology
Information
Abstract
BACKGROUND: The incidence of Amaranthaceae pollen allergy has increased due to the desertification occurring in many countries. In some regions of Spain, Salsola kali is the main cause of pollinosis, at almost the same level as olive and grass pollen. Sal k 1 - the sensitization marker of S. kali pollinosis - is used in clinical diagnosis, but is purified at a low yield from pollen. We aimed to produce a recombinant (r)Sal k 1 able to span the structural and immunological properties of the natural isoforms from pollen, and validate its potential use for diagnosis. METHODS: Specific cDNA was amplified by PCR, cloned into the pET41b vector and used to transform BL21 (DE3) Escherichia coli cells. Immunoblotting, ELISA, basophil activation and skin-prick tests were used to validate the recombinant protein against Sal k 1 isolated from pollen. Sera and blood cells from S. kali pollen-sensitized patients and specific monoclonal and polyclonal antisera were used. RESULTS: rSal k 1 was produced in bacteria with a yield of 7.5 mg/l of cell culture. The protein was purified to homogeneity and structural and immunologically validated against the natural form. rSal k 1 exhibited a higher IgE cross-reactivity with plant-derived food extracts such as peanut, almond or tomato than with pollen sources such as Platanus acerifolia and Oleaceae members. CONCLUSIONS: rSal k 1 expressed in bacteria retains intact structural and immunological properties in comparison to the pollen-derived allergen. It spans the immunological properties of most of the isoforms found in pollen, and it might substitute natural Sal k 1 in clinical diagnosis.
International
Si
JCR
Si
Title
International Archives of Allergy And Immunology
ISBN
1018-2438
Impact factor JCR
2,433
Impact info
Volume
167
Journal number
From page
83
To page
93
Month
SIN MES
Ranking
Participants
  • Autor: Salvador Mas (Departamento de Bioquímica y Biología Molecular I, Universidad Complutense de Madrid,)
  • Autor: Patrice Boissy (ALK-Abelló S.A)
  • Autor: Rafael I Monsalve (ALK-Abelló S.A)
  • Autor: Javier Cuesta-Herranz (Hospital Fundación Jiménez Díaz, Madrid)
  • Autor: Araceli Diaz Perales (UPM)
  • Autor: Javier Fernández (Departamento Medicina Clínica, Universidad Miguel Hernández de Elche, Alicante , and)
  • Autor: Carlos Colás (Hospital Clínico Universitario `Lozano Blesa¿, Zaragoza , Spain)
  • Autor: Rosalía Rodríguez (Departamento de Bioquímica y Biología Molecular I, Universidad Complutense de Madrid)
  • Autor: Rodrigo Barderas (Departamento de Bioquímica y Biología Molecular I, Universidad Complutense de Madrid)
  • Autor: Mayte Villalba (Departamento de Bioquímica y Biología Molecular I, Universidad Complutense de Madrid)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Biotecnología Vegetal
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)