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Memorias de investigación
Ponencias en congresos:
TEMPERATURE AND VOLTAGE COUPLING TO TRPM8 CHANNEL OPENING
Año:2015
Áreas de investigación
  • Fisiología,
  • Biología estructural,
  • Biología celular,
  • Biología molecular
Datos
Descripción
Expressed in somatosensory neurons of the dorsal root and trigeminal ganglion, the transient receptor potential melastatin 8 (TRPM8) channel is a Ca2+-permeable cation channel activated by cold, voltage, PIP2 and menthol. Although TRPM8 channel gating has been characterized at the single channel and macroscopic current levels, there is currently no consensus regarding the extent to which temperature and voltage sensors couple to the conduction gate. In the present study we extended the range of voltages at which the TRPM8-induced ionic currents were measured and made careful measurements of the maximum open probability the channel can attain at different temperatures by means of fluctuation analysis. The first direct measurements of TRPM8 channel temperature-driven conformational rearrangements provided here suggest that temperature alone is able to open the channel and that the opening reaction is voltage-independent. Voltage is a partial activator of TRPM8 channels, since absolute open probability values measured with fully activated voltage sensors are less than 1 and they decrease as temperature rises. By unveiling the fast temperature-dependent deactivation process, we show that TRPM8 channel deactivation is well described by a double exponential time course. The fast and slow deactivation processes are temperature-dependent with enthalpy changes of 27.2 kcalmol-1 and 30.8 kcalmol-1. The overall Q10 for the closing reaction is about 33. A three-tiered allosteric model containing four voltage sensors and four temperature sensors can account for the complex deactivation kinetics and coupling between voltage and temperature sensor activation and channel opening.
Internacional
Si
Nombre congreso
Biophysical Society 59th Annual Meeting
Tipo de participación
960
Lugar del congreso
Baltomore, Maryland. United State
Revisores
Si
ISBN o ISSN
0006-3495
DOI
10.1016/j.bpj.2014.11.233
Fecha inicio congreso
07/02/2015
Fecha fin congreso
11/02/2015
Desde la página
37
Hasta la página
38
Título de las actas
TEMPERATURE AND VOLTAGE COUPLING TO TRPM8 CHANNEL OPENING
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Natalia Raddatz . (UPM)
  • Autor: Juan Pablo Castillo (CINV, Universidad de Valparaiso, Valparaiso, Chile)
  • Autor: Carlos Gonzalez (CINV, Universidad de Valparaiso, Valparaiso, Chile)
  • Autor: Osvaldo Alvarez (Universidad de Chile, Santiago, Chile)
  • Autor: Ramón Latorre (CINV, Universidad de Valparaiso, Valparaiso, Chile)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
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