Descripción
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Expressed in somatosensory neurons of the dorsal root and trigeminal ganglion, the transient receptor potential melastatin 8 (TRPM8) channel is a Ca2+-permeable cation channel activated by cold, voltage, PIP2 and menthol. Although TRPM8 channel gating has been characterized at the single channel and macroscopic current levels, there is currently no consensus regarding the extent to which temperature and voltage sensors couple to the conduction gate. In the present study we extended the range of voltages at which the TRPM8-induced ionic currents were measured and made careful measurements of the maximum open probability the channel can attain at different temperatures by means of fluctuation analysis. The first direct measurements of TRPM8 channel temperature-driven conformational rearrangements provided here suggest that temperature alone is able to open the channel and that the opening reaction is voltage-independent. Voltage is a partial activator of TRPM8 channels, since absolute open probability values measured with fully activated voltage sensors are less than 1 and they decrease as temperature rises. By unveiling the fast temperature-dependent deactivation process, we show that TRPM8 channel deactivation is well described by a double exponential time course. The fast and slow deactivation processes are temperature-dependent with enthalpy changes of 27.2 kcalmol-1 and 30.8 kcalmol-1. The overall Q10 for the closing reaction is about 33. A three-tiered allosteric model containing four voltage sensors and four temperature sensors can account for the complex deactivation kinetics and coupling between voltage and temperature sensor activation and channel opening. | |
Internacional
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Si |
Nombre congreso
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Biophysical Society 59th Annual Meeting |
Tipo de participación
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960 |
Lugar del congreso
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Baltomore, Maryland. United State |
Revisores
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Si |
ISBN o ISSN
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0006-3495 |
DOI
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10.1016/j.bpj.2014.11.233 |
Fecha inicio congreso
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07/02/2015 |
Fecha fin congreso
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11/02/2015 |
Desde la página
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37 |
Hasta la página
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38 |
Título de las actas
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TEMPERATURE AND VOLTAGE COUPLING TO TRPM8 CHANNEL OPENING |