Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Effect of antioxidants on the genetic stability of cryopreserved mint shoot tips by encapsulation?dehydration
Year:2016
Research Areas
  • Plants and animal biology and ecology
Information
Abstract
The effect of antioxidants applied in one step of a cryopreservation protocol by encapsulation?dehydration on recovery and genetic stability of mint shoot tips has been studied. Glutathione (0.16 or 0.24 mM), ascorbic acid (0.28 or 0.43 mM) and ?-tocopherol (vitamin E) were added to the preculture medium (0.3 M sucrose). DNA was extracted from three different types of samples: leaves from shoots, callus at the base of shoots and callus. RAPD and AFLP markers were used to assess the genetic stability. The use of antioxidants did not improve recovery after cryopreservation. One of the genotypes, ?MEN 198?, showed higher percentage of stable samples than the other one, ?MEN 186? (56 vs. 37 %; considering all treatments and types of explant). The use of vitamin E improved the percentage of stable samples with respect to control treatment (no antioxidant) in ?MEN 186?. No differences in the percentages of stable samples were observed among cryopreserved and non-cryopreserved (treated similarly without immersion in liquid nitrogen) plant material. Recovered shoots of both genotypes showed higher stability (76?80 % stable samples) than callus samples (14?22 %).
International
Si
JCR
Si
Title
Plant Cell, Tissue and Organ Culture
ISBN
0167-6857
Impact factor JCR
Impact info
Volume
127
10.1007/s11240-016-1056-8
Journal number
From page
359
To page
368
Month
SIN MES
Ranking
Participants
  • Autor: M. Elena Gonzalez Benito (UPM)
  • Autor: Carolina Kremer
  • Autor: Miguel Angel Ibañez Ruiz (UPM)
  • Autor: Maria Carmen Martin Fernandez (UPM)
Research Group, Departaments and Institutes related
  • Creador: Departamento: Biotecnología - Biología Vegetal
  • Departamento: Economía Agraria, Estadística y Gestión de Empresas
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)