Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Chicken embryo lethality assay for determining the lethal dose and virulence of E. faecalis.
Research Areas
  • Poultry farming,
  • Animal production,
  • Veterinary medicine,
  • Bacterial pathogens of animals
Enterococcus faecalis is the major pathogen found in field cases of amyloid arthropathy in chickens. Given the need of a better understanding of the virulence mechanisms of the causa-tive strains, the Embryo Lethality Assay (ELA) is proposed in the present study as a model to evaluate the virulence of E. faecalis strains, specifically the pathogenic avian strain K923/96, which was previously related with amyloid arthropathy. Hence, 0.2 ml of five doses of the cited strain (from 2.5 to 2500 colony-forming unit (CFU) per ml) were inoculated into the allantoic cavity of 10 day-old embryos. The embryo mortality rate (EMR) was determined by daily candling of the eggs over a period of 7 days and based on this information the median lethal dose (LD50) was calculated. The ELA was repeated four times on a sample of 3443 eggs. The infectious dose showed a significant effect on the EMR. The EMR with the doses of 2.5, 5, 25, 250 and 2500 CFU/ml was 43%, 45%, 63%, 90% and 93% respectively. The estimated dose at LD50 was 6.6 CFU/ml. As expected, the higher the infectious dose, the greater the EMR and the lower the embryo survival time. The highest EMR was recorded after three and four days post inoculation in all doses. In conclusion, these results can be used as a basis for further researches on the E. faecalis virulence. In order to corroborate its model capacity to predict the virulence of this bacterium, more ELA with different E. faecalis strains are required.
Avian Pathology
Impact factor JCR
Impact info
Datos JCR del año 2013
Journal number
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  • Autor: Ana e Blanco Garcia (UPM)
  • Autor: Martin Barz (Lohmann Tierzucht GmbH)
  • Autor: Wiebke Icken (Lohmann Tierzucht GmbH)
  • Autor: David Cavero (Lohmann Tierzucht GmbH)
  • Autor: A. Reza Sharifi (Georg-August-University Goettingen)
  • Autor: Matthias Voss (Lohmann Tierzucht GmbH)
  • Autor: Rudolf Preisinger (Lohmann Tierzucht GmbH)
  • Autor: Carlos Isidro Buxade Carbo (UPM)
Research Group, Departaments and Institutes related
  • Creador: Departamento: Producción Agraria
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