Observatorio de I+D+i UPM

Memorias de investigación
Characterization of endosymbiotic bacteria of the endemism Astragalus nitidiflorus
Research Areas
  • Natural sciences and health sciences
Astragalus nitidiflorus is an endangered and endemic legume of SE Spain. After was considered extinct, it was re-discovered in 2004 and later included in a governmental conservation program. The main objective of the project is the reintroduction and maintenance of the A. nitidiflorus. According to this purpose, the isolation and characterization of its endosymbionts has been proposed for the development of bacterial bioinoculants that improve the survivor rate of the reintroduced plants. However, the isolation of the A. nitidiflorus symbiont (Ans) was not successful in the normal rhizobial growing media. PCR amplification of recA gene was performed from nodule DNA and showed that the putative Ans inhabiting the A. nitidiflorus nodules probably correspond to a Mesorhizobium sp. Two different strategies are carried out to overcome the non-culturable status of Ans: (1) direct isolation from Cartagena soils and (2) indirect isolation using relative legumes as alternative hosts.
Mark Rating
  • Director: Luis Rey Navarro (UPM)
  • Autor: Alba Pacheco (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: BIOLOGÍA MOLECULAR Y COMPUTACIONAL
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)