Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
Copper transport to Medicago truncatula nodules is mediated by MtCOPT1
Year:2016
Research Areas
  • Natural sciences and health sciences
Information
Abstract
Copper is a common redox cofactor for many physiological processes in plants, including photosynthesis, mitochondrial respiration, and symbiotic nitrogen fixation (SNF). The latter is the conversion, fixation, of N2 into NH3, a process carried out by endosymbiotic bacteria (rhizobia) in legume root nodules. SNF requires relatively large amounts of copper as an essential cofactor of some of the key reactions being carried out by the endosymbiotic rhizobia (Preisig et al., 1996). This oligonutrient is delivered by the host legume through the vasculature to the nodules and released in the infection/differentiation zone of them (where rhizobia differentiate into nitrogen-fixing bacteroids). From there, a plasma membrane transporter has to introduce copper into the cell for further cuproprotein assembly (Rodríguez-Haas et al., 2013). COPT family of copper transporters, mediate high affinity copper transport into the cytosol of eukaryotic cells (Peñarrubia et al., (2015); Sancenón et al., (2003)). From the eight COPT family genes present in M. truncatula genome, MtCOPT1 is the only one induced specifically in nodule. MtCOPT1 is able to restore Saccharomyces cerevisae ?ctr1 (defective in copper uptake) capacity to transport copper. Inmunolocalization and GUS fusion studies localize MtCOPT1 mainly in the plasma membrane of cells in the infection/differenciation zone of the nodule. In addition, a Tnt-1-derived knockdown mutant line for MtCOPT1 shows decreased nitrogenase activity when compared with the wild-type line. This activity is, at least, partially rescued when a wild-type copy of MtCOPT1 gene is reintroduced. Taken together, these data suggest an important role of MtCOPT1 copper-mediated transport for SNF.
International
No
Congress
XIII REUNIÓN DE BIOLOGÍA MOLECULAR DE PLANTAS
970
Place
Reviewers
Si
ISBN/ISSN
0000000000
Start Date
22/06/2016
End Date
24/06/2016
From page
161
To page
161
XIII REUNIÓN DE BIOLOGÍA MOLECULAR DE PLANTAS
Participants
  • Autor: Marta Senovilla Ramos (UPM)
  • Autor: M del Rosario Castro Rodriguez (UPM)
  • Autor: Juan Imperial Ródenas (UPM)
  • Autor: Manuel Gonzalez Guerrero (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: BIOLOGÍA MOLECULAR Y COMPUTACIONAL
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
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