Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Differential modulation of plant immune responses by diverse 2 members of the Pseudomonas savastanoi pv. savastanoi HopAF 3 type III effector family
Year:2016
Research Areas
  • Molecular, cellular and genetic biology,
  • Agriculture
Information
Abstract
The Pseudomonas savastanoi pv. savastanoi NCPPB 3335 type III secretion system (T3SS) effector repertoire includes 33 candidates, seven of which translocate into host cells and interfere with plant defences. The present study was performed to investigate the co-existence of both plasmid- and chromosomalencoded members of the HopAF effector family, HopAF1-1 and HopAF1-2, respectively, in the genome of NCPPB 3335. Here, we show that the HopAF1 paralogues are widely distributed in the Pseudomonas syringae complex, where HopAF1-1 is most similar to the homologues encoded by other P. syringae pathovars infecting woody hosts that belong to phylogroups 1 and 3. We show that the expression of both HopAF1-1 and HopAF-2 is transcriptionally dependent on HrpL and demonstrate their delivery into Nicotiana tabacum leaves. Although the heterologous delivery of either HopAF1-1 or HopAF1-2 significantly suppressed the production of defence-associated reactive oxygen species levels, only HopAF1-2 reduced the levels of callose deposition. Moreover, the expression of HopAF1-2 by functionally effectorless P. syringae pv. tomato DC3000D28E completely inhibited the hypersensitive response in tobacco and significantly increased the competitiveness of the strain in Nicotiana benthamiana. Despite their functional differences, subcellular localization studies reveal that green fluorescent protein (GFP) fusions to either HopAF1-1 or HopAF1-2 are targeted to the plasma membrane when they are expressed in plant cells, a process that is completely dependent on the integrity of their N-myristoylation motif. Our results further support the notion that highly similar T3SS effectors might differentially interact with diverse plant targets, even when they co-localize in the same cell compartment.
International
Si
JCR
Si
Title
Molecular Plant Pathology
ISBN
1464-6722
Impact factor JCR
4,335
Impact info
Volume
10.1111/mpp.12420
Journal number
on-line
From page
on -line
To page
on-line
Month
SIN MES
Ranking
D1 (16/209 Plant Sciences)
Participants
  • Autor: M.Pilar Castañeda-Ojeda (Universidad de Málaga)
  • Autor: Emilia Antonia Lopez Solanilla (UPM)
  • Autor: Cayo Ramos (Universidad de Málaga)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Interacciones Moleculares Planta-Patógeno
  • Departamento: Biotecnología - Biología Vegetal
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)