Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
Next-Generation Sequencing Applied to Pollen and Fungal Identification in the Atmosphere of Madrid
Year:2016
Research Areas
  • Quality of the environment,
  • Molecular biology,
  • Microbiology,
  • Palaeobotany,
  • Biodiversity characterisation,
  • Biotechnology,
  • Atmospheric pollution
Information
Abstract
Pollen and fungal spores monitoring in urban environments has an important clinical interest because of their potential for causing allergy-related diseases: asthma, rhinitis, atopic eczema, etc. Moreover, some opportunistic fungi can develop dangerous diseases, especially to immunocompromised individuals, such as aspergillosis or fungal pneumonia. We have studied the fungal and pollen communities from two different sampling sites in the city of Madrid (Spain) during different seasons using both traditional (microscopy) and Next-Generation DNA Sequencing (NGS) methods. The results obtained for fungal determination show that high-throughput sequencing yields much greater diversity than microscopy analyses, identifying groups and species that are hardly detected or identified by morphology. Moreover, Ascomycetes (and Capnodiales within this group) are the most abundant fungi at all seasons analyzed according to both NGS and traditional methods. An increment in Basidiomycetes abundance was also detected from winter to spring time. In regard to pollen determination, the outcome from NGS technology strongly correlates with the results from morphological identification, allowing us to detect the noticeable change of pollen communities from winter season (peak of Pinales) to spring (peak of Fagales). Again, sequence-based analyses can recognize a wider diversity but some technical adjustments are necessary to reach precisely the taxonomic level of species. Altogether, our results show that NGS technologies are a promising alternative for pollen and fungal identification in aerobiology, although more studies comparing traditional and NGS methods are still needed in order to obtain accurate results.
International
Si
Congress
6th European Symposium on Aerobiology of the European Aerobiology Society
960
Place
Lyon (Francia)
Reviewers
Si
ISBN/ISSN
Start Date
18/07/2016
End Date
22/07/2016
From page
151
To page
151
Abstract book ESA 2016
Participants
  • Autor: Andrés Nuñez Hernández (UPM)
  • Autor: Guillermo Amo (Facultad de Farmacia (Universidad Complutense de Madrid))
  • Autor: Zuzana Ferencova (Facultad de Farmacia (Universidad Complutense de Madrid))
  • Autor: Alberto Rastrojo (Centro de Biología Molecular Severo Ochoa (CBMSO-CSIC))
  • Autor: Ana Maria Garcia Ruiz (UPM)
  • Autor: A. Montserrat Gutiérrez-Bustillo (Facultad de Farmacia (Universidad Complutense de Madrid))
  • Autor: Antonio Alcamí (Centro de Biología Molecular Severo Ochoa (CBMSO-CSIC))
  • Autor: Diego Alejandro Moreno Gomez (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Bioingeniería y Materiales (BIO-MAT)
  • Departamento: Física Aplicada e Ingeniería de Materiales
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