Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
A Developmental Switch of Gene Expression in the Barley Seed Mediated by HvVP1 (Viviparous-1) and HvGAMYB Interactions
Year:2016
Research Areas
  • Molecular, cellular and genetic biology,
  • Plants and animal biology and ecology
Information
Abstract
The accumulation of storage compounds in the starchy endosperm of developing cereal seeds is highly regulated at the transcriptional level. These compounds, mainly starch and proteins, are hydrolyzed upon germination to allow seedling growth. The transcription factor HvGAMYB is a master activator both in the maturation phase of seed development and upon germination, acting in combination with other transcription factors. However, the precise mechanism controlling the switch from maturation to germination programs remains unclear. We report here the identification and molecular characterization of Hordeum vulgare VIVIPAROUS1 (HvVP1), orthologous to ABA-INSENSITIVE3 from Arabidopsis thaliana. HvVP1 transcripts accumulate in the endosperm and the embryo of developing seeds at early stages and in the embryo and aleurone of germinating seeds up to 24 h of imbibition. In transient expression assays, HvVP1 controls the activation of Hor2 and Amy6.4 promoters exerted by HvGAMYB. HvVP1 interacts with HvGAMYB in Saccharomyces cerevisiae and in the plant nuclei, hindering its interaction with other transcription factors involved in seed gene expression programs, like BPBF. Similarly, this interaction leads to a decrease in the DNA binding of HvGAMYB and the Barley Prolamine-Box binding Factor (BPBF) to their target sequences. Our results indicate that the HvVP1 expression pattern controls the full Hor2 expression activated by GAMYB and BPBF in the developing endosperm and the Amy6.4 activation in postgerminative reserve mobilization mediated by GAMYB. All these data demonstrate the participation of HvVP1 in antagonistic gene expression programs and support its central role as a gene expression switch during seed maturation and germination.
International
Si
JCR
Si
Title
Plant Physiology
ISBN
0032-0889
Impact factor JCR
6,28
Impact info
Volume
170
10.1104/pp.16.00092
Journal number
From page
2146
To page
2158
Month
SIN MES
Ranking
Plant Physiology se encuentra en la posición 8 de 209 en el área de "Plant Sciences", por tanto sde encuentra en el 5% superior del ranking.
Participants
  • Autor: Zamira Abraham
  • Autor: Raquel Iglesias Fernandez (UPM)
  • Autor: Manuel Martinez Muñoz (UPM)
  • Autor: Ignacio Rubio Somoza
  • Autor: M. Isabel Diaz Rodriguez (UPM)
  • Autor: Pilar Carbonero Zalduegui (UPM)
  • Autor: Jesus Vicente Carbajosa (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Redes Reguladoras del Desarrollo y Respuesta a Estrés en Plantas
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)