Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Identification of the ligand of Pru p 3, a peach LTP
Year:2017
Research Areas
  • Molecular, cellular and genetic biology
Information
Abstract
The allergen Pru p 3, a peach lipid transfer protein, has been well studied. However, its physiological function remains to be elucidated. Our results showed that Pru p 3 usually carries a lipid ligand that play an essential role in its function in plants. Using ESI-qToF, we observed that the ligand was a derivative of camptothecin binding to phytosphingosine, wich that is inserted into the hydrophobic tunnel of the protein. In addition, the described ligand displayed topoisomerase I activity inhibition and self-fluorescence, both recognized as camptothecin properties. During flower development, the highest expression of Pru p 3 was detected in the styles of pollinated flowers, in contrast to its non-expression in unpollinated pistils, where expression decreased after anthesis. During ripening, the expression of Pru p 3 were observed mainly in peel but not in pulp. In this sense, Pru p 3 protein was also localized in trichomes covering the fruit epidermis.
International
Si
JCR
No
Title
Plant Molecular Biology
ISBN
0167-4412
Impact factor JCR
Impact info
Volume
10.1007/s11103-017-0590-z
Journal number
From page
1
To page
12
Month
SIN MES
Ranking
Participants
  • Autor: Nuria Cubells Baeza (UPM)
  • Autor: Cristina Gómez Casado (CEU San Pablo)
  • Autor: Leticia Tordesillas (Icahn School of Medicine at Mount Sinai)
  • Autor: Maria del Carmen Ramirez Castillejo (UPM)
  • Autor: Maria Garrido Arandia (UPM)
  • Autor: Pablo Gonzalez-Melendi De Leon (UPM)
  • Autor: Luis Fernandez Pacios (UPM)
  • Autor: Araceli Diaz Perales (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Interacciones moleculares planta-insecto.
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)