Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Mechanisms underlying induction of allergic sensitization by Pru p 3
Year:2017
Research Areas
  • Molecular, cellular and genetic biology
Information
Abstract
The lipid transfer protein Pru p 3 is one of the most common plant food allergens among adults in Southern Europe with a prevalence that has been increasing particularly in children. LTP allergy is frequently life-long and is also one of the causes of anaphylactic reactions that are potentially life-threatening. However, the origin of its immunological activity is unknown.The nature of the lipid-ligand of Pru p 3 has recently been identified as a derivative of the alkaloid camptothecin linked to a phytosphingosine tail. Taking into account that lipids can induce immune responses, in this work we have evaluated the immunogenic activity of this lipid-ligand using a range of in vitro models (moDC, PBMCs, THP1 and epithelial cells as Caco2), and a mouse model of anaphylaxis to peach. We have determined that the lipid-ligand of Pru p 3 could act as an adjuvant with the ability to modulate the immune system presumably toward a Th2 response characteristic of allergy as it has been demonstrated in a mouse model of anaphylaxis. Moreover, the lipid-ligand of Pru p 3 co-localized to CD1d in epithelial cells (Caco2) and interacted to Cd1d tetramers to activate iNKT of the immune system.
International
Si
JCR
Si
Title
Clinical And Experimental Allergy
ISBN
0954-7894
Impact factor JCR
5,587
Impact info
Datos JCR del año 2015
Volume
10.1111/cea.12962
Journal number
From page
1
To page
10
Month
SIN MES
Ranking
Participants
  • Autor: Leticia Tordesillas (Icahn School of Medicine at Mount Sinai)
  • Autor: Nuria Cubells Baeza (UPM)
  • Autor: Cristina Gómez-Casado (CEU San Pablo University)
  • Autor: Vanesa Esteban (Fundación Jimenez Díaz)
  • Autor: Weronika Barcik (swiss institute allergy asthma research (SIAF))
  • Autor: Liam O'Mahony (swiss institute allergy asthma research (SIAF))
  • Autor: Maria del Carmen Ramirez Castillejo (UPM)
  • Autor: Luis Fernandez Pacios (UPM)
  • Autor: Maria Garrido Arandia (UPM)
  • Autor: Araceli Diaz Perales (UPM)
Research Group, Departaments and Institutes related
  • Creador: Departamento: Biotecnología - Biología Vegetal
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)