Observatorio de I+D+i UPM

Memorias de investigación
Book chapters:
Characterization of Plant Cell Wall Damage-Associated Molecular Patterns Regulating Immune Responses
Year:2017
Research Areas
  • Molecular, cellular and genetic biology,
  • Plant biotechnology
Information
Abstract
The plant cell wall is one of the first defensive barriers that pathogens need to overcome to successfully colonize plant tissues. Plant cell wall is considered a dynamic structure that regulates both constitutive and inducible defense mechanisms. The wall is a potential source of a diverse set of damage-associated molecular patterns (DAMPs), which are signalling molecules that trigger immune responses. However, just a few active wall ligands, like oligogalacturonic acids (OGs), have been characterised so far. To identify additional wall-derived DAMPs, we obtained different plant wall fractions and tested their capacity to trigger immune responses using a calcium read-out system. To characterise the active DAMPs structures present in these fractions, we applied Glycome Profiling, a technology that uses a large and diverse set of specific monoclonal antibodies against wall carbohydrate ligands. The methods describe here can be used in combination with other biochemical approaches to identify and purify new plant cell wall DAMPs.
International
Si
10.1007/978-1-4939-6859-6_2
Book Edition
Book Publishing
Springer Science+Business Media LLC
ISBN
978-1-4939-6859-6
Series
Book title
Plant Pattern Recognition Receptors
From page
13
To page
23
Participants
  • Autor: Laura Bacete Cano (UPM)
  • Autor: Hugo Melida Martinez (UPM)
  • Autor: Sivakumar Pattathil (Complex Carbohydrate Research Center (CCRC), University of Georgia, Athens, GA 30602, U.S.A)
  • Autor: Michael G. Hahn (Complex Carbohydrate Research Center (CCRC), University of Georgia, Athens, GA 30602, U.S.A)
  • Autor: Antonio Molina Fernandez (UPM)
  • Autor: Eva Miedes Vicente (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Interacciones Moleculares Planta-Patógeno
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)