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Descripción
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| At early stages of fruit development, cells actively divide and following this phase a period of cell expansion where fruit elongates occurs. Both processes imply the action of several cell wall remodelling enzymes (CWRE), such as endo-?-mannanases (MAN; EC. 3.2.1.78). The AtMAN7 gene is abundantly expressed at the early and middle stages of fruit development (Early Green and Late Green, respectively), dropping at the final stage (Brown). Interestingly, the transcript accumulation of the basic leucine zipper transcription factor (TF) AtbZIP44, previously described as a transcriptional activator of AtMAN7 upon seed germination (Iglesias-Fernández et al., 2013), shows a similar expression profile. The transcriptional combinatorial network through which AtbZIP44 regulates the AtMAN7 gene expression during silique development has been explored in the yeast 2-hybrid system (Y2H), using as a bait AtbZIP44 and as a prey, an arrayed yeast library of circa 1,200 TF Open Reading Frames (ORFs) from Arabidopsis thaliana (Castrillo et al., 2011). The interaction between AtbZIP44 (S1-group of bZIPs) and AtbZIP9 (C-group) has been established and further corroborated by Bimolecular Fluorescence Complementation (BiFC) assays in bombarded onion epidermal cells. The physiological significance of this interaction upon silique development is investigated by molecular techniques such as RT-qPCR analyses of AtMAN7 gene expression in AtbZIP44 and AtbZIP9 T-DNA insertion mutants, and by qualitative ?-glucuronidase (GUS) hystochemical assays in PAtMAN7::uidA, PAtbZIP44::uidA and PAtbZIP9::uidA transgenic Arabidopsis lines. | |
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Internacional
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Si |
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Nombre congreso
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Workshop New Frontiers in Plant Biology |
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Tipo de participación
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970 |
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Lugar del congreso
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Centro de Biotecnología y Genómica de Plantas |
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Revisores
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Si |
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ISBN o ISSN
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0000-0000 |
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DOI
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Fecha inicio congreso
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15/06/2016 |
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Fecha fin congreso
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17/06/2016 |
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Desde la página
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19 |
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Hasta la página
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19 |
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Título de las actas
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A role for the interaction between AtbZIP44 and AtbZIP9 in the transcriptional regulation of Arabidopsis thaliana silique development |