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Descripción
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| Stable and high expression of transgenes is essential for the development of genetically engineered crops. The high variability amongst transgenic lines due to gene silencing, joined to the low expression of transgenes, are nowadays a big challenge for plant synthetic biology and plant molecular farming. RNAi pathways guided by dsRNAs are responsible for transgene silencing. Even though the events that occur after the silencing is established are very well known, the steps that initiate this response remain unknown. We have evidence that different transgenes licence different silencing responses, even when located in the same region of the host genome. In particular, promoters seem to have a key role on the recognition of exogenous DNA by the plant and initiating a stronger or weaker response. We have generated a tool that allows us to measure transgene silencing by measuring luciferase expression and activity, therefore, establishing a correlation between LUC expression and transgene silencing. This assay provides a sensitive method for the accurate quantification of small changes in transcription and translation resulting from changes in transcriptional and post-transcriptional gene silencing. By using this tool, we are analysing a set of Arabidopsis thaliana promoters with different features, to discover the signals responsible for the recognition of transgenes by the plant. After these analyses, we expect to clarify the signals involved in the licensing of transgene silencing. This will help avoid silencing in biotechnological applications, making possible to obtain higher yields of recombinant protein production and simplifying the complexity of gene expression regulation for synthetic biology and crop improvement. | |
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Internacional
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Si |
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Nombre congreso
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Workshop New Frontiers in Plant Biology |
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Tipo de participación
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970 |
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Lugar del congreso
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Centro de Biotecnología y Genómica de Plantas |
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Revisores
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Si |
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ISBN o ISSN
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0000-0000 |
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DOI
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Fecha inicio congreso
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15/06/2016 |
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Fecha fin congreso
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17/06/2016 |
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Desde la página
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29 |
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Hasta la página
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29 |
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Título de las actas
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Gene Silencing in Plant Biotechnological Applications |