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Descripción
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| Copper is an essential nutrient for symbiotic nitrogen fixation (SNF), the conversion of N2 into NH3 carried out by endosymbiotic rhizobia within legume root nodules. Among multiple processes, copper is required for fending off free radicals or to provide energy in the microaerobic environment within the nodule (Preisig et al., 1996). Once copper is in the plant, it is delivered by the vasculature to the apoplast of the infection zone of the nodule. From there, a plasma membrane transporter has to introduce this nutrient into the cell for copper-protein assembly (Rodríguez-Haas et al., 2013). Candidates for this role ought to be member of the COPT family of copper transporters, since they mediate high affinity copper transport into the cytosol of eukaryotic cells (Peñarrubia et al., 2015; Sancenón et al., 2003). From the eight COPT family genes present in M. truncatula genome, MtCOPT1 is the only one induced specifically in nodule. MtCOPT1 is able to restore Saccharomyces cerevisae ?ctr1 (defective in copper uptake) capacity to transport copper. Inmunolocalization and GUS fusion studies localize MtCOPT1 mainly in the zone of the nodule where metals are transported from the apoplast into the host cell cytoplasm. In addition, a Tnt-1-derived knockdown mutant line for MtCOPT1 shows decreased nitrogenase activity when compared with the wild-type line. This activity is, at least, partially rescued when a wild-type copy of MtCOPT1 gene is reintroduced. Taken together, all these data suggest an important role of MtCOPT1 copper-mediated transport for SNF. | |
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Internacional
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Si |
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Nombre congreso
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Workshop New Frontiers in Plant Biology |
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Tipo de participación
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970 |
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Lugar del congreso
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Centro de Biotecnología y Genómica de Plantas |
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Revisores
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Si |
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ISBN o ISSN
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0000-0000 |
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DOI
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Fecha inicio congreso
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15/06/2016 |
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Fecha fin congreso
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17/06/2016 |
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Desde la página
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37 |
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Hasta la página
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37 |
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Título de las actas
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MtCOPT1 mediates copper transport to Medicago truncatula nodules |