Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Ectopic Expression of CDF3 Genes in Tomato Enhances Biomass Production and Yield under Salinity Stress Conditions
Year:2017
Research Areas
  • Molecular, cellular and genetic biology
Information
Abstract
Cycling Dof Factor (CDF) transcription factors (TFs) are involved in multiple processes related to plant growth and development. A member of this family, CDF3, has recently been linked in Arabidopsis to the regulation of primary metabolism and abiotic stress responses, but its role in crop production under stress is still unknown. In this study, we characterized tomato plants overexpressing the CDF3 genes from Arabidopsis and tomato and analyzed their effects on growth and yield under salinity, additionally gaining deeper insights into the molecular function of these TFs. Our results provide evidence for higher biomass production and yield in the 35S::AtCDF3 and 35S::SlCDF3 plants, likely due to a higher photosynthetic capacity resulting in increased sucrose availability. Transcriptome analysis revealed that CDF3 genes regulate a set of genes involved in redox homeostasis, photosynthesis performance and primary metabolism that lead to enhanced biomass production. Consistently, metabolomic profiling revealed that CDF3 evokes changes in the primary metabolism triggering enhanced nitrogen assimilation, and disclosed that the amount of some protective metabolites including sucrose, GABA and asparagine were higher in vegetative tissues of CDF3 overexpressing plants. Altogether these changes improved performance of 35S::AtCDF3 and 35S::SlCDF3 plants under salinity conditions. Moreover, the overexpression of CDF3 genes modified organic acid and sugar content in fruits, improving variables related to flavor perception and fruit quality. Overall, our results associate the CDF3 TF with a role in the control of growth and C/N metabolism, and highlight that overexpression of CDF3 genes can substantially improve plant yield.
International
Si
JCR
Si
Title
Frontiers in Plant Science
ISBN
1664-462X
Impact factor JCR
4,495
Impact info
Datos JCR del año 2015
Volume
10.3389/fpls.2017.00660
Journal number
8
From page
660
To page
660
Month
MAYO
Ranking
Participants
  • Autor: Begoña Renau-Morata (Universitat Politècnica de València)
  • Autor: Rosa-Victoria Molina (Universitat Politècnica de València)
  • Autor: Laura Carrillo Gil (UPM)
  • Autor: Jaime Cebolla-Cornejo (Universitat Politècnica de València)
  • Autor: Manuel Sánchez-Perales (Universitat Politècnica de València)
  • Autor: Stephan Pollmann (UPM)
  • Autor: Jose Domingo Dominguez Figueroa (UPM)
  • Autor: Alba Rocio Corrales Ducuara (UPM)
  • Autor: Jaume Flexas (Universitat de les Illes Balears)
  • Autor: Jesus Vicente Carbajosa (UPM)
  • Autor: Joaquin Medina Alcazar (UPM)
  • Autor: Sergio G. Nebauer (Universitat Politècnica de València)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Redes Reguladoras del Desarrollo y Respuesta a Estrés en Plantas
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)