Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Validation of the Hirst-Type Spore Trap for Simultaneous Monitoring of Prokaryotic and Eukaryotic Biodiversities in Urban Air Samples by Next-Generation Sequencing
Year:2017
Research Areas
  • Molecular biology,
  • Microbiology,
  • Palaeobotany,
  • Biodiversity characterisation,
  • Biotechnology,
  • Quality of the environment,
  • Atmospheric pollution
Information
Abstract
Pollen, fungi, and bacteria are the main microscopic biological entities present in outdoor air, causing allergy symptoms and disease transmission and having a significant role in atmosphere dynamics. Despite their relevance, a method for monitoring simultaneously these biological particles in metropolitan environments has not yet been developed. Here, we assessed the use of the Hirst-type spore trap to characterize the global airborne biota by high-throughput DNA sequencing, selecting regions of the 16S rRNA gene and internal transcribed spacer for the taxonomic assignment. We showed that aerobiological communities are well represented by this approach. The operational taxonomic units (OTUs) of two traps working synchronically compiled >87% of the total relative abundance for bacterial diversity collected in each sampler, >89% for fungi, and >97% for pollen. We found a good correspondence between traditional characterization by microscopy and genetic identification, obtaining more-accurate taxonomic assignments and detecting a greater diversity using the latter. We also demonstrated that DNA sequencing accurately detects differences in biodiversity between samples. We concluded that high-throughput DNA sequencing applied to aerobiological samples obtained with Hirst spore traps provides reliable results and can be easily implemented for monitoring prokaryotic and eukaryotic entities present in the air of urban areas.
International
Si
JCR
Si
Title
Applied And Environmental Microbiology
ISBN
0099-2240
Impact factor JCR
3,807
Impact info
Volume
83
10.1128/AEM.00472-17
Journal number
13
From page
e00472-17
To page
14
Month
ABRIL
Ranking
33/160 Biotechnology & Applied Microbiology
Participants
  • Autor: Andrés Nuñez Hernández (UPM)
  • Autor: Guillermo Amo de Paz (Facultad de Farmacia (Universidad Complutense de Madrid))
  • Autor: Zuzana Ferencova (Facutad de Farmacia (Universidad Complutense de Madrid))
  • Autor: Alberto Rastrojo (Centro de Biología Molecular Severo Ochoa (CBMSO-CSIC))
  • Autor: Raúl Guantes (Facultad de Ciencias (Universidad Autónoma de Madrid))
  • Autor: Ana Maria Garcia Ruiz (UPM)
  • Autor: Antonio Alcamí (Centro de Biología Molecular Severo Ochoa (CBMSO-CSIC))
  • Autor: A. Montserrat Gutiérrez-Bustillo (Facultad de Farmacia (Universidad Complutense de Madrid))
  • Autor: Diego Alejandro Moreno Gomez (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Bioingeniería y Materiales (BIO-MAT)
S2i 2019 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)