Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
Assessment of geometry in 2D immune systems using high accuracy laser-based bioprinting techniques
Year:2017
Research Areas
  • Physical aplications to problems and biological systems,
  • Mechanical engineering,
  • Laser devices
Information
Abstract
The immune system is a very complex system that comprises a network of genetic and signaling pathways subtending a network of interacting cells. The location of the cells in a network, along with the gene products they interact with, rules the behavior of the immune system. Therefore, there is a great interest in understanding properly the role of a cell in such networks to increase our knowledge of the immune system response. In order to acquire a better understanding of these processes, cell printing with high spatial resolution emerges as one of the promising approaches to organize cells in two and three-dimensional patterns to enable the study the geometry influence in these interactions. In particular, laser assisted bio-printing techniques using sub-nanosecond laser sources have better characteristics for application in this field, mainly due to its higher spatial resolution, cell viability percentage and process automation. This work presents laser assisted bio-printing of antigen-presenting cells (APCs) in two-dimensional geometries, placing cellular components on a matrix previously generated on demand, permitting to test the molecular interactions between APCs and lymphocytes; as well as the generation of two-dimensional structures designed ad hoc in order to study the mechanisms of mobilization of immune system cells. The use of laser assisted bio-printing, along with APCs and lymphocytes emulate the structure of different niches of the immune system so that we can analyse functional requirement of these interaction.
International
Si
Congress
Photonics West 2017
960
Place
San Francisco, EE.UU.
Reviewers
Si
ISBN/ISSN
9781510605657
10.1117/12.2253622
Start Date
28/01/2017
End Date
02/02/2017
From page
1
To page
1
Proc. SPIE 10062, Optical Interactions with Tissue and Cells XXVIII
Participants
  • Autor: Sara Lauzurica Santiago (UPM)
  • Autor: A. Márquez (UPM)
  • Autor: Carlos Luis Molpeceres Alvarez (UPM)
  • Autor: L. Notario (Centro Nacional de Microbiología-ISCIII)
  • Autor: M. Gómez-Fontela (Centro Nacional de Microbiología-ISCIII)
  • Autor: P. Lauzurica (Centro Nacional de Microbiología-ISCIII)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Manufactura Avanzada con Láser
  • Departamento: Física Aplicada e Ingeniería de Materiales
  • Centro o Instituto I+D+i: Centro Laser
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)