Observatorio de I+D+i UPM

Memorias de investigación
Advances in engineering eukaryotic nitrogenase
Research Areas
  • Microbiology,
  • Biotechnology
The generation of cereal plants able to express nitrogenase, and thus of utilizing atmospheric nitrogen, has the potential of changing agriculture systems worldwide. Nitrogenases are two-component enzymes consisting of a dinitrogenase and a dinitrogenase reductase, which in the case of the most abundant and catalytically efficient molybdenum nitrogenase are called MoFe protein and Fe protein, respectively. The immediate obstacles to engineer nitrogenase expression in plants and other eukaryotic organisms are its sensitivity towards O2 and its biochemical complexity, since it requires a large number of genetic parts to function optimally. Nitrogenase genetic engineering can be accomplished out by putting together four distinct genetic modules: the Fe protein module, the MoFe protein module, the FeMo-cofactor biosynthetic module, and the electron transport module. Here we use yeast as model organism and a combination of synthetic biology and biochemical complementation assays to explore the functionality of these modules in eukaryotic organisms.
Entity Nationality
Sin nacionalidad
  • Autor: Luis Manuel Rubio Herrero (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: BIOLOGÍA MOLECULAR Y COMPUTACIONAL
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)