Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
HOP3, a member of the HOP family in Arabidopsis, interacts with BiP and plays a major role in the ER stress response.
Year:2017
Research Areas
  • Plants and animal biology and ecology
Information
Abstract
HSP70-HSP90 organizing protein (HOP) is a well-studied family of cytosolic cochaperones. However, the possible role of HOP during the endoplasmic reticulum (ER) stress response and the identity of its interactors within the ER were not previously addressed in any eukaryote. We have demonstrated that Arabidopsis HOP3, whose function was not studied before, interacts in vivo with cytosolic HSP90 and HSP70, and, unexpectedly, with binding immunoglobulin protein (BiP), a HSP70 ER-resident protein. Although BiP lacks the domain described in other eukaryotes for HOP-HSP70 binding, it interacts with HOP3 through a non-canonical association to its nucleotide binding domain. Consistent with this interaction with BiP, HOP3 is partially localized at the ER. Moreover, HOP3 is induced both at transcript and protein levels by unfolded protein response (UPR) inducer agents by a mechanism dependent on inositol-requiring enzyme 1 (IRE1). Importantly, hop3 loss-of-function mutants show a reduction in pollen germination and a hypersensitive phenotype in the presence of ER stress inducer agents, a phenotype that is reverted by the addition of the chemical chaperone tauroursodeoxycholic acid (TUDCA). All these data demonstrate, for the first time in any eukaryote, a main role of HOP as an important regulator of the ER stress response, a process intimately linked in plants to important specific developmental programs and to environmental stress sensing and response.
International
Si
JCR
Si
Title
Plant, Cell & Environment
ISBN
0140-7791
Impact factor JCR
Impact info
Area: Plant science
Volume
10.1111/pce.12927
Journal number
From page
1341
To page
1355
Month
AGOSTO
Ranking
(10/209) 1º cuartil
Participants
  • Autor: Nuria Fernandez Bautista-Abad (UPM)
  • Autor: Lourdes Fernández Calvino (UPM)
  • Autor: Alfonso Muñoz Gutiérrez (UPM)
  • Autor: Mar Castellano Moreno (UPM)
Research Group, Departaments and Institutes related
  • Creador: Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)