Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
INSIGHTS INTO LIGHT AND ABA-GA RESPONSES DURING SEED GERMINATION.
Year:2018
Research Areas
  • Natural sciences and health sciences,
  • Molecular biology
Information
Abstract
Seed germination is a crucial process in spermatophytes with an impact on crop establishment and yield. This process depends on hormonal regulation, being abscisic acid (ABA) and gibberellins (GAs) key regulators playing antagonistic roles. Several environmental factors may compromise seed germination and plant growth, mainly through the regulation of ABA-GA metabolism and signalling pathways (Colebrook et al., 2014). By screening a seed library (Weiste et al., 2007) we have identified Arabidopsis lines constitutively overexpressing the single transcription factor LID1 (light-in-darkness1) with improved germination under conditions known to decrease GA biosynthesis (paclobutrazol; PAC). Whereas this phenotype is compatible with enhanced GA levels/signalling or reduced ABA sensitivity, the overexpression lines (LID1oex) show reduced germination in the presence of ABA and drought-like conditions (high mannitol levels) known to increase ABA levels. Loss-of-function lid1 mutants show opposite phenotypes, with reduced germination in the presence of PAC and enhanced germination in ABA and mannitol, thus pointing to a possible role of LID1 in GA-ABA crosstalk. Light is known to influence ABA-GA balance and growth (de Lucas et al., 2008; Feng et al., 2008; Oh et al., 2009), and lid1 mutants germinate in the dark more rapidly than WT seeds. Under light conditions, they also germinate and develop into photomorphogenic seedlings more quickly than WT plants. Molecular studies indicate that LID1 is expressed specifically in the seed, is a transcriptional repressor and interacts with DELLA proteins. Our molecular studies indicate that LID1 is a novel regulator integrating light and growth responses in seeds with a promising biotechnological potential.
International
Si
Congress
XIV Reunión de Biología Molecular de Plantas
970
Place
Salamanca
Reviewers
Si
ISBN/ISSN
0000-0000
Start Date
04/07/2018
End Date
06/07/2018
From page
137
To page
137
0000-0000
Participants
  • Autor: Gerardo Carrera Castaño (UPM)
  • Autor: Rocío Sánchez Montesinos (UPM)
  • Autor: Christoph Weiste (Julius-Maximillians-Universität Würzburg)
  • Autor: Wolfgang Dröge-Laser (Julius-Maximillians-Universität Würzburg)
  • Autor: Luis Gomez Fernandez (UPM)
  • Autor: Luis Oñate Sanchez (UPM)
Research Group, Departaments and Institutes related
  • Creador: Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
  • Departamento: Sistemas y Recursos Naturales
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