Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
LID1 is a novel regulator involved in light signaling with an impact on growth responses and ABA-GA crosstalk.
Year:2018
Research Areas
  • Natural sciences and health sciences,
  • Molecular biology
Information
Abstract
Seed vigour is associated with reliable and successful crop establishment and yield under fluctuating environmental cues. Environmental factors which show relevant seasonal fluctuations, such as light, temperature and water availability, compromise plant growth and negatively affect seed vigour traits, in part by reducing GA levels and signalling (1). By screening a seed library (2) we have identified Arabidopsis lines constitutively overexpressing the single transcription factor LID1 (light-in-darkness1) with improved germination under conditions known to decrease GA biosynthesis (paclobutrazol; PAC). Whereas this phenotype is compatible with enhanced GA levels/signalling or reduced ABA sensitivity, the overexpression lines (LID1oex) show reduced germination in the presence of ABA and drought-like conditions (high mannitol levels) known to increase ABA levels. Loss-of-function lid1 mutants show opposite phenotypes, with reduced germination in the presence of PAC and enhanced germination in ABA and mannitol, thus pointing to a possible role of LID1 in GA-ABA crosstalk. Light is known to influence ABA-GA balance and growth (3-5), and lid1 mutants germinate in the dark more rapidly than WT seeds. Under light conditions, they also germinate and develop into photomorphogenic seedlings more quickly than WT plants. Molecular studies indicate that LID1 is expressed specifically in the seed, is a transcriptional repressor and interacts with DELLA proteins. Our molecular studies indicate that LID1 is a novel regulator integrating light and growth responses in seeds with a promising biotechnological potential.
International
No
Congress
Workshop "Frontiers in Plant Biology" 2018
970
Place
Pozuelo de Alarcón, Madrid
Reviewers
Si
ISBN/ISSN
0000-0000
Start Date
30/05/2018
End Date
01/06/2018
From page
7
To page
7
0000-0000
Participants
  • Autor: Gerardo Carrera Castaño (UPM)
  • Autor: Rocío Sánchez Montesinos (UPM)
  • Autor: Christoph Weiste (Julius-Maximillians-Universität Würzburg)
  • Autor: Wolfgang Dröge-Laser (Julius-Maximillians-Universität Würzburg)
  • Autor: Luis Gomez Fernandez (UPM)
  • Autor: Luis Oñate Sanchez (UPM)
Research Group, Departaments and Institutes related
  • Creador: Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
  • Departamento: Sistemas y Recursos Naturales
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)