Observatorio de I+D+i UPM

Memorias de investigación
Book chapters:
Screening Arrayed Libraries with DNA and Protein Baits to Identify Interacting Proteins
Year:2018
Research Areas
  • Molecular, cellular and genetic biology,
  • Plants and animal biology and ecology
Information
Abstract
Molecular interactions are an integral part of the regulatory mechanisms controlling gene expression. The yeast one- and two-hybrid systems (Y1H/Y2H) have been widely used by many laboratories to detect DNA?protein (Y1H) and protein?protein interactions (Y2H). The development of efficient cloning systems have promoted the generation of large open reading frame (ORF) clone collections (libraries) for several organisms. Functional analyses of such large collections require the establishment of adequate protocols. Here, we describe a simple straightforward procedure for high-throughput screenings of arrayed libraries with DNA or protein baits that can be carried out by one person with minimal labor and not requiring robotics. The protocol can also be scaled up or down and is compatible with several library formats. Procedures to make yeast stocks for long-term storage (tube and microplate formats) are also provided.
International
Si
10.1007/978-1-4939-7871-7_9
Book Edition
2018
Book Publishing
Springer Protocols; Humana Press, New York, NY
ISBN
978-1-4939-7870-0
Series
Book title
Two-Hybrid Systems. Methods in Molecular Biology, vol 1794.
From page
131
To page
149
Participants
  • Autor: Rocío Sánchez Montesinos (UPM)
  • Autor: Luis Oñate Sanchez (UPM)
Research Group, Departaments and Institutes related
  • Creador: Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)