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Descripción
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| Botrytis cinerea is a necrotrophic plant pathogenic fungus that causes enormous economic losses worldwide in important crops including fruit trees and horticultural or ornamental plants. The discovery of mycoviruses and their use as a tool to increase the fungal knowledge, as well as their possible application as biocontrol agents, has encouraged its study in B. cinerea. In Spain, B. cinerea is a pathogenic fungus of several economically important crops; however, no studies on the presence of mycoviruses are reported. We have analyzed this matter to get a better understanding of the interactions among virus, fungi and plants in B. cinerea pathosystems. Several B. cinerea isolates were collected in a survey from commercial greenhouses of six common winter crops (tomato, pepper, cucumber, aubergine, bean and zucchini) located in Southeastern Spain. In order to analyze the presence of extrachromosomal genetic elements, nucleic acid purification was performed. In every extraction, fungal genomic DNA and ribosomal RNA were visualized. We also detected extra genomic elements in every host but aubergine, some of them disappeared after a RNasa A treatment, in high- and low-ionic strength buffers, indicating that probably correspond to single strand (ss) RNA molecules. Some of the extrachromosomal elements correspond to double strand (ds) RNA molecules since were digested by RNasa A in low-ionic strength buffer but not in high-ionic strength buffer. Since most of mycoviruses described contain a dsRNA genome, dsRNA was purified by CF11 cellulose chromatography. We detected extrachromosomal elements corresponding to dsRNA molecules in every host analyzed, including aubergine. The nature of these molecules was confirmed by degradation with RNasa A in a low-ionic strength buffer and DNasa. The number (1-5 extrachromosomal genetic elements), relative concentration and sizes (approach 0.7 to more than 13 kb) were variable between isolates. These results confirm the existence of mycovirus like elements in Spanish isolates of B. cinerea with different compositions. Characterization of these putative mycovirus are currently under progress. | |
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Internacional
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Si |
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ISSN o ISBN
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0000-0000 |
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Entidad relacionada
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4th International Mycovirus Symposium, Universidad de Agricultura de Huazhong |
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Nacionalidad Entidad
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CHINA |
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Lugar del congreso
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Wuhan, P. R. China |