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Memorias de investigación
Communications at congresses:
Mitogen-activated protein kinase phosphatase 1 (MKP1) negatively regulates Microbe-Associated Molecular Pattern-triggered immunity in Arabidopsis.
Year:2018
Research Areas
  • Molecular, cellular and genetic biology
Information
Abstract
In the search for early signalling components of Microbe-Associated Molecular Pattern (MAMP)-triggered immunity we identified a null allele of the mitogen-activated protein kinase phosphatase 1 gene (MKP1). MKP1 appears as a negative regulator of broad MAMP-triggering immunity responses since mkp1 mutant is more resistant to the necrotrophic fungus Plectosphaerella cucumerina BMM (PcBMM), the hemibiotrophic bacterium Pseudomonas syringae pv. tomato DC3000 and the oomycete Hyaloperonospora arbidopsidis. MKP1 regulates ROS production by modulating the NADPH oxidase RBOHD, since mkp1 plants constitutively overexpressing RbohD (35S::RbohD mkp1) display elevated ROS levels upon MAMP treatment. A metabolomic analysis reveals a significant reprograming of the metabolic profile in mkp1, with more than 200 metabolites showing differential accumulation compared to wild-type plants. Antimicrobial compounds of the glucosinolate pathway or camalexin as well as defense-associated metabolites, like saliciylic acid (SA) are among the compounds that mkp1 plants accumulate at high levels. To characterize the elements responsible for this broad enhanced resistance, we crossed mkp1 mutant to lines compromised in production of tryptophan derived metabolites and SA. Patho-tests performed in the combinatory mutants revealed that distinct elements are required for the enhanced resistance to the different pathogens.
International
No
Congress
XIII Congreso de Biología Molecular de Plantas
970
Place
Salamanca
Reviewers
Si
ISBN/ISSN
000-00-0000-000-0
Start Date
04/07/2018
End Date
06/07/2018
From page
224
To page
224
Abstracts
Participants
  • Autor: Miguel Angel Torres Lacruz (UPM)
  • Autor: Viviana Pamela Escudero Welsch (UPM)
  • Autor: M Delgado
  • Autor: Sara Sopeña-Torres
  • Autor: Sanjai Swami (UPM)
  • Autor: Jorge Morales (UPM)
  • Autor: Antonio Muñoz Barrios (UPM)
  • Autor: H Mélida
  • Autor: Lucia Jorda Miro (UPM)
  • Autor: Antonio Molina Fernandez (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: BIOLOGÍA MOLECULAR Y COMPUTACIONAL
  • Grupo de Investigación: Patología Vegetal
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
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