Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
Replication of viruses in hosts different from the natural host
Research Areas
  • Virology,
  • Crop production
Mycoviruses or fungal viruses are widespread in fungi, and to date have been isolated from various fungi, including mushrooms, plant-pathogenic and medical fungi. The origin and evolution of mycoviruses have been explained with two main hypotheses: the ancient coevolution and the plant virus hypotheses. The plant virus hypothesis proposes that at least the plant-pathogenic fungi have acquired recently the viruses from its infected plant host. This hypothesis is based on the similarity between fungal and plant viruses with the important difference that no mycovirus encodes a movement protein and some of them not even a coat protein. Therefore, if fungal viruses have evolved from plant viruses, they have eliminated genes encoding proteins dispensable for their survival inside the fungal host. If micoviruses derive from adaptation of plant viruses to fungal hosts, as proposed by the plant virus hypothesis, could the mycovirus leave the fungal host and infect plants again? If the answer is yes, could this mycovirus create by recombination with other viruses a new plant virus? In favor of the hypothesis that plant viruses can evolve by reassortments of different genome segments from viruses infecting fungi and plants, is the discovery of the plant virus genus Ourmiavirus. In this work we analyzed the replication in plants of two different mycoviruses. In the first approach we have used viral RNA transcribed in vitro from an infectious clone, and in the second approach we have used viral particles of a mycovirus naturally encapsidated. The assays have been developed in protoplasts and plants of the host Nicotiana benthamiana. The preliminary results indicated that mycoviruses can replicate in protoplasts of N. benthamiana, but the replication cannot be maintained in inoculated leaves of plants of the same host. Experiments are ongoing in new hosts to analyze the role of this factor in the failure of mycoviral replication
XIX Congreso de la Sociedad Española de Fitopatología
Start Date
End Date
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Libro de resúmenes
  • Autor: Carlos García-Benítez (UPM)
  • Autor: Eva Álvarez-Medrano (UPM)
  • Autor: Manuel Guillermo Moreno-Pérez (UPM)
  • Autor: Yeturu Sivaprasad (UPM)
  • Autor: Livia Donaire (UPM)
  • Autor: Maria Angeles Ayllon Talavera (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Patología Vegetal
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
S2i 2021 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)