Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
The evolution of host-range: genetic mechanisms associated to the acquisition of a new host by a plant virus
Year:2018
Research Areas
  • Virology,
  • Crop production
Information
Abstract
The acquisition of new hosts, or host range expansion, provides a pathogen with more opportunities for transmission and survival, but may be limited by across-host fitness trade-offs. A major cause of across-host trade-offs in viruses is antagonistic pleiotropy, that is, different phenotypic effects of mutations in different hosts, a Genotype x Environment interaction. In addition, epistasis (a Genotype x Genotype interaction) also may play a role in generating across-host fitness trade-offs. We have analysed if there are fitness trade-offs, and their cause, associated to the acquisition of a new host by a plant virus, Tobacco mild green mosaic virus (TMGMV), which infects pepper and Nicotiana glauca in SE Spain. Worldwide, TMGMV has been reported in N. glauca since the early XX century, while in pepper only since the 1980s, so that pepper can be considered the new host. TMGMV accumulation level differed in both hosts, and the assay of field isolates in the homologous and heterologous hosts showed adaptation of N. glauca isolates, but not of pepper isolates, to their original host, with asymmetrical fitness trade-offs. The role of the amino acid at two dimorphic positions in the coat protein, and of a duplication in the 3?UTR, in the differential multiplication in pepper and N. glauca was analysed in the collection of field isolates and by mutagenesis of cDNA clones derived from two TMGMV isolates. Results showed host-specific effects of the analysed mutations on virus multiplication, which in addition depended on the genetic background. Thus, both pleiotropy and epistasis, and their interaction, play a role in TMGMV host-range evolution.
International
No
Congress
XIX Congreso de la Sociedad Española de Fitopatología
960
Place
Toledo
Reviewers
Si
ISBN/ISSN
000-00-0000-000-0
Start Date
08/10/2018
End Date
10/10/2018
From page
43
To page
43
Libro de resúmenes
Participants
  • Autor: Sayanta Bera . (UPM)
  • Autor: Aurora Fraile Perez (UPM)
  • Autor: Fernando Garcia-Arenal Rodriguez (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Patología Vegetal
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
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