Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
The evolution of host-range: genetic mechanisms associated to the acquisition of a new host by a plant virus
Research Areas
  • Virology,
  • Crop production
The acquisition of new hosts, or host range expansion, provides a pathogen with more opportunities for transmission and survival, but may be limited by across-host fitness trade-offs. A major cause of across-host trade-offs in viruses is antagonistic pleiotropy, that is, different phenotypic effects of mutations in different hosts, a Genotype x Environment interaction. In addition, epistasis (a Genotype x Genotype interaction) also may play a role in generating across-host fitness trade-offs. We have analysed if there are fitness trade-offs, and their cause, associated to the acquisition of a new host by a plant virus, Tobacco mild green mosaic virus (TMGMV), which infects pepper and Nicotiana glauca in SE Spain. Worldwide, TMGMV has been reported in N. glauca since the early XX century, while in pepper only since the 1980s, so that pepper can be considered the new host. TMGMV accumulation level differed in both hosts, and the assay of field isolates in the homologous and heterologous hosts showed adaptation of N. glauca isolates, but not of pepper isolates, to their original host, with asymmetrical fitness trade-offs. The role of the amino acid at two dimorphic positions in the coat protein, and of a duplication in the 3?UTR, in the differential multiplication in pepper and N. glauca was analysed in the collection of field isolates and by mutagenesis of cDNA clones derived from two TMGMV isolates. Results showed host-specific effects of the analysed mutations on virus multiplication, which in addition depended on the genetic background. Thus, both pleiotropy and epistasis, and their interaction, play a role in TMGMV host-range evolution.
XIX Congreso de la Sociedad Española de Fitopatología
Start Date
End Date
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Libro de resúmenes
  • Autor: Sayanta Bera . (UPM)
  • Autor: Aurora Fraile Perez (UPM)
  • Autor: Fernando Garcia-Arenal Rodriguez (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Patología Vegetal
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)