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Descripción
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| Over 30% of plant viruses are seed transmitted. However, the genetic determinants of seed transmission are poorly understood. Viruses reach the seed by: (i) infection of ovules or pollen, and (ii) embryo invasion before the programmed cell death of the embryonic suspensor. Hence, for seed transmission to occur the virus must reach plant reproductive organs before gametogenesis and/or while embryonic suspensor is still functional. Thus, virus genetic determinants of seed transmission would control virus multiplication and movement. We have analyzed whether the level of virus multiplication and the speed of within-host invasion determine seed transmission rate, using Cucumber mosaic virus (CMV) and its natural host Arabidopsis thaliana. We conducted a time course infection experiment, in which six arabidopsis accessions were challenged against CMV genotypes with high/low seed transmission rates. The level of virus multiplication in plant growth and reproductive structures was monitored every two days along the plant lifespan, and seed transmission rate was estimated. In parallel, we compared the genomic sequences of the utilized CMV genotypes. Results indicated that higher CMV multiplication leads to higher seed transmission, and that adaptation to this mode of transmission is linked to mutations in the 3¿ UTR of the viral genome. | |
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Internacional
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Si |
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ISSN o ISBN
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000-00-0000-000-0 |
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Entidad relacionada
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Nacionalidad Entidad
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FRANCIA |
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Lugar del congreso
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Montpelier |