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ZF1 and ZF3 are important regulatory components of the nitrogen and carbon metabolism of Arabidopsis thaliana
Year:2018
Research Areas
  • Biological sciences
Information
Abstract
Nitrogen is an essential macronutrient whose availability in soil is crucial for plant growth and crop productivity. Nitrate is the main source of inorganic nitrogen for land plants. However, it is usually limiting in the majority of the ecosystems, due to a variety of biotic and abiotic factors like microbial consumption, water leaching and soil erosion. Besides a macronutrient, nitrate is also a signal molecule that regulates the expression of a variety of genes that modulate many aspects of metabolism, growth and development. Here we identify the roles of Arabidopsis Transcription factors Cycling DOF Factor 1 and 3 (CDF1 and CDF3) in nitrate responses. Loss-of-function null mutant cdf3-1 and cdf1-R exhibit nitrate-dependent root phenotypes, whereas CDF1 and CDF3 overexpression lines showed increased biomass and altered root architecture. Transcriptomic analyses of overexpressor lines indicated that both factors regulate the expression an important set of carbon and nitrogen regulated genes. Consistently, metabolite profiling disclosed that the total amount of key nitrogen metabolites including glutamate and glutamine were higher in CDF1/3-overexpressing plants in N-sufficient and limiting conditions. By contrast, cdf3-1 and cdf1-R mutants exhibited lower amounts of those metabolites. In tomato, the overexpression of CDF3 gene modified amino acid and sugar content, improving biomass and yield. These results identify CDF1 and CDF3 as important regulatory factors of the nitrate response in Arabidopsis, suggesting that they are potential targets for improving nutrient use efficiency in crops.
International
Si
Congress
XI REUNION NACIONAL METABOLISMO DEL NITROGENO
970
Place
Segovia, España
Reviewers
Si
ISBN/ISSN
0000-0000
Start Date
16/05/2018
End Date
18/05/2018
From page
0
To page
0
XI REUNION NACIONAL METABOLISMO DEL NITROGENO
Participants
  • Autor: Jesus Vicente Carbajosa (UPM)
  • Autor: Joaquin Medina Alcazar (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Redes Reguladoras del Desarrollo y Respuesta a Estrés en Plantas
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
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