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Descripción
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| In an effort to identify and characterize minimal versions of transit peptides (mTPs) that work in crops, we have cloned four previously described TPs from the first methionine up to the predicted cleavage site by the Stromal Processing Peptidase, fused to the eGFP. For cloning, we adapted the MoClo system using a pUC57 vector backbone and the MoClo cloning cassette ligated within, optimizing for plant transformation via particle bombardment. The resulting constructs were used for stable transformation of Oryza sativa embryos. Embryogenic calli were recovered from the bombarded embryos 2 weeks later by hygromycin B selection. We have analyzed the expression of eGFP by immunoblot analysis, confirming that several mTPs work in targeting protein accumulation to the rice calli pro-plastid. For further experiments to determine mTPs effectiveness in targeting protein accumulation to mature chloroplasts, regeneration of rice plants is being carried out. | |
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Internacional
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Si |
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Nombre congreso
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3rd International Conference on ?Plant Cells & Tissue in Vitro? |
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Tipo de participación
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960 |
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Lugar del congreso
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Viena |
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Revisores
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Si |
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ISBN o ISSN
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DOI
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Fecha inicio congreso
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01/07/2019 |
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Fecha fin congreso
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02/07/2019 |
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Título de las actas
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