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Descripción
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| The type 6 secretion systems (T6SSs) are protein secretion systems identified in more than 25% of Gram-negative bacteria, mostly within the Proteobacterium Phylum (Bernal et al, 2018). These systems work as nanosyringes that translocate proteins called effectors from bacterial cytoplasm directly to eukaryotic or to other bacterial cells or to the external milieu. Numerous functions have been described for the effectors, mainly as antibacterial toxins, virulence factors against eukaryotic hosts and metal transport (Coulthurst, 2019). The majority of T6SSs require 13 conserved proteins encoded by clustered genes called tss. The T6SS gene clusters code for structural proteins of the nanosyringe, accessory and regulatory proteins, effectors, and the so called immunity proteins that counteract toxic effectors. The genes that code the effector and immunity proteins are found in pairs called EI pairs. At the present is not much known about T6SS effectors in rhizobia and their role in symbiosis. This work shows that the T6SS of Rhizobium etli Mim1 (ReMim1) has a positive role in symbiosis with common beans. Conserved genes of T6SS have been identified in two divergent operons of 14-gene each in pRetMIM1f plasmid. Immunodetection of TssD protein, a conserved key structural component of T6SS systems also known as Hcp, indicated that ReMim1 T6SS is active at high cell densities, in the presence of root exudates, and in bean nodules. Three different mutants affected in T6SS structural genes produced plants with lower dry weight and smaller nodules than the wild-type strain, indicating for the first time that the T6SS plays a positive role in Rhizobium?legume symbiosis (Salinero et al, 2019). | |
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Internacional
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Si |
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Nombre congreso
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XVII National Meeting of the Spanish Society of Nitrogen Fixation VI Portuguese-Spanish Congress on Nitrogen Fixation |
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Tipo de participación
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960 |
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Lugar del congreso
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Madrid |
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Revisores
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Si |
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ISBN o ISSN
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978-84-8344-705-5 |
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DOI
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Fecha inicio congreso
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10/06/2019 |
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Fecha fin congreso
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12/06/2019 |
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Desde la página
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55 |
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Hasta la página
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55 |
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Título de las actas
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Biological Nitrogen Fixation and Plant-Associated Microorganisms |