Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Metabolic fingerprinting allows discrimination between Ulmus pumila and U. minor, and between U. minor clones of different susceptibility to Dutch elm disease
Year:2008
Research Areas
  • Genetics,
  • Crop production
Information
Abstract
Experiments were conducted to test whether Fourier transform-infrared (FT-IR) spectroscopy, coupled with chemometric methods, can distinguish healthy xylem tissues collected from elms known to differ significantly in their susceptibility to Ophiostoma novo-ulmi Brasier. Twig samples from resistant Ulmus pumila L., susceptible U. minor Mill. and resistant U. minor clones were harvested on 1 May, 15 May, 1 June, 1 July and 1 September 2004, and subjected to FT-IR analysis. The application of principal component analysis to the spectral data, followed by discriminant function analysis, discriminated between the three groups of samples in all harvesting dates. The examination of the DFloading plots allowed the identification of key regions within the spectra for the separation between clusters. The chemical assignments of these key regions allowed the following interpretations to be made: (i) U. pumila tissues contained enhanced levels of starch, cellulose and lignin with respect to U. minor tissues and (ii) resistant U. minor tissues contained enhanced levels of starch, cellulose and pectic polysaccharides with respect to susceptible U. minor tissues. The possible roles of the compositional differences in disease resistance, as well as the potential use of FT-IR spectroscopy and chemometrics as a tool for screening resistant elms are discussed.
International
Si
JCR
Si
Title
FOREST PATHOLOGY
ISBN
1437-4781
Impact factor JCR
0,744
Impact info
Volume
38
Journal number
0
From page
244
To page
256
Month
ENERO
Ranking
Participants
  • Participante: MA Coimbra (Universidade de Aveiro, Portugal)
  • Autor: Luis Alfonso Gil Sanchez (UPM)
  • Participante: Alejandro Solla (Universidad de Extremadura)
  • Autor: Juan Antonio Martin Garcia (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Genética y Fisiología Forestal
  • Departamento: Silvopascicultura
S2i 2019 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)