Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Analysis of Spanish populations of Ophiostoma ulmi and O. novo-ulmi using phenotypic characteristics and RAPD markers
Year:2008
Research Areas
  • Genetics,
  • Crop production
Information
Abstract
One hundred and six isolates of the Dutch elm disease (DED) fungi Ophiostoma ulmi and Ophiostoma novo-ulmi were collected from elm trees with symptoms in 15 regions of Spain. Isolates were compared with eight reference strains belonging to O. ulmi and the two subspecies of O. novo-ulmi. The purpose of this study was to assign Spanish isolates to species and subspecies of the DED fungi and to analyse the genetic variability within the Spanish populations of these pathogens. Isolates were examined for their growth rates, colony morphologies and fertility responses and by using random amplified polymorphic DNA (RAPD) markers. Six isolates were identified as O. ulmi , 16 as O. novo-ulmi ssp. novo-ulmi and 78 as O. novo-ulmi ssp. americana . Nei’s and Shannon’s diversity indices and the upgma dendrogram from RAPD profiles indicated a high level of variation among isolates, probably reflecting post-epidemic status of DED in Spain. Although most isolates were separated into three major clusters representing the three taxa of DED fungi in the RAPD analysis, two isolates from central Spain clustered between O. novo-ulmi ssp. americana and O. novo-ulmi ssp. novo-ulmi , and four isolates from Mallorca clustered between O. ulmi and the group representing O. novo-ulmi ssp. novo-ulmi . Mating tests conducted with these isolates revealed a variety of fertility responses. The novel combinations between molecular profile and fertility reaction suggest that three isolates from Mallorca may be interspecific hybrids of the DED fungi.
International
Si
JCR
Si
Title
PLANT PATHOLOGY
ISBN
0032-0862
Impact factor JCR
2,012
Impact info
Volume
57
10.1111/j.1365-3059.2007.01692.x
Journal number
0
From page
33
To page
44
Month
FEBRERO
Ranking
Participants
  • Participante: Alejandro Solla (Universidad de Extremadura, Spain)
  • Participante: M Hubbes (University of Toronto)
  • Autor: Luis Alfonso Gil Sanchez (UPM)
  • Participante: M. Carmen Dacasa (Universidad Politécnica de Madrid, Spain)
  • Participante: C Nasmith (University of Toronto, Canada)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Genética y Fisiología Forestal
  • Departamento: Silvopascicultura
S2i 2019 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)