Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
High sensitive label-free nano-biosensor
Year:2008
Research Areas
  • Polymers,
  • Electronics engineering,
  • Mechanical engineering,
  • Magnetic materials
Information
Abstract
Label-free integrated optical biosensors have been demonstrated to be very sensitive biosensors presenting advantages as high sensitivity, small size, and high scale integration. Recently, it has been reported an integrated photonic sensor based on a slotwaveguide resonator (Figure 1a). Theorethical calculations show that the most sensitive area of the device is the inside of the slot, being able to detect mass changes of 0.8 pg/mm2, due to the variation in the refraction index the recognition antigen-antibody. Thus, an important challenge is to develop chemical methods that let the immobilisation of bioreceptors in a robust and oriented manner selectively on the nitride guides (figure 1a), and keeping their bioavailability. Our studies employing planar test structures demonstrated that the reaction between the amine groups on the silicon nitride surface and a well known cross-linker, glutaraldehyde, provided the selective immobilisation of the bioreceptors against silicon oxyde. In this work, we employed the optimized immobilisation strategy to demonstrate the detection of label-free molecular binding reactions on the surface of a slot-waveguide ring resonator. Bovine serum albumin (BSA) protein and anti-BSA are used to study the biosensor performance. Figure 1b and 1c illustrates the resonante wavelength shift as a function of anti-BSA and BSA concentration. The estimated limits of detection of the device for anti-BSA and BSA molecules were 28 and 16 pg/mm2, limited by wavelength resolution. These values favorable compare with those of state-of-the-art integrated photonic biosensors based on conventional waveguides.
International
Si
Congress
II Workshop of Nanoscience and Analytical Nanotechnology
960
Place
Tarragona
Reviewers
Si
ISBN/ISSN
0000000000
Start Date
25/09/2008
End Date
26/09/2008
From page
1
To page
1
ABSTRACTS
Participants
  • AUTOR: A. MAQUIEIRA (Instituto de Química Molecular Aplicada, Departamento de Química, Universidad Politécnica de Valencia)
  • AUTOR: M.V. GONZÁLEZ-PEDRO (Instituto de Química Molecular Aplicada, Departamento de Química, Universidad Politécnica de Valencia)
  • Autor: Miguel Holgado Bolaños (UPM)
  • AUTOR: R. PUCHADES (Instituto de Química Molecular Aplicada, Departamento de Química, Universidad Politécnica de Valencia)
  • Autor: Carlos Angulo Barrios (UPM)
  • AUTOR: M.J. BAÑULS (Instituto de Química Molecular Aplicada, Departamento de Química, Universidad Politécnica de Valencia)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Ingeniería y Aplicaciones del Láser
  • Centro o Instituto I+D+i: Centro Laser
  • Centro o Instituto I+D+i: Instituto Universitario de Sistemas Optoelectrónicos y Microtecnología
  • Departamento: Física Aplicada a la Ingeniería Industrial
  • Grupo de Investigación: Grupo de Dispositivos Semiconductores del ISOM
S2i 2019 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
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