Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
High diversity and mophological convergence among melanised fungi from rock formations in the Central Mountain System of Spain
Year:2008
Research Areas
  • Microbiology
Information
Abstract
Melanised fungi were isolated from rock surfaces in the Central Mountain System of Spain. Two hundred sixty six isolates were recovered from four geologically and topographically distinct sites. Microsatellite-primed PCR techniques were used to group isolates into genotypes assumed to represent species. One hundred and sixty three genotypes were characterised from the four sites. Only five genotypes were common to two or more sites. Morphological and molecular data were used to characterise and identify representative strains, but morphology rarely provided a definitive identification due to the scarce differentiation of the fungal structures or the apparent novelty of the isolates. Vegetative states of fungi prevailed in culture and in many cases could not be reliably distinguished without sequence data. Morphological characters that were widespread among the isolates included scarce micronematous conidial states, endoconidia, mycelia with dark olive-green or black hyphae, and mycelia with torulose, isodiametric or moniliform hyphae whose cells develop one or more transverse and/or oblique septa. In many of the strains, mature hyphae disarticulated, suggesting asexual reproduction by a thallic micronematous conidiogenesis or by simple fragmentation. Sequencing of the internal transcribed spacers (ITS1, ITS2) and 5.8S rDNA gene were employed to investigate the phylogenetic affinities of the isolates. According to ITS sequence alignments, the majority of the isolates could be grouped among four main orders of Pezizomycotina: Pleosporales, Dothideales, Capnodiales, and Chaetothyriales. Ubiquitous known soil and epiphytic fungi species were generally absent from the rock surfaces. In part, the mycota of the rock surfaces shared similar elements with melanised fungi from plant surfaces and fungi described from rock formations in Europe and Antarctica. The possibility that some of the fungi were lichen mycobionts or lichen parasites could not be ruled out.
International
Si
JCR
Si
Title
PERSOONIA
ISBN
0031-5850
Impact factor JCR
1,1
Impact info
Volume
21
10.3767/003158508X371379
Journal number
2
From page
93
To page
110
Month
DICIEMBRE
Ranking
Participants
  • Participante: Gonzalo Platas (Merck, Sharp & Dohme de España, S.A.)
  • Autor: Constantino Ruibal Villaseñor (UPM)
  • Participante: Gerald F. Bills (Merck, Sharp & Dohme de España, S.A.)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Grupo de Inv. en Bioingeniería y Materiales (BIO-MAT)
  • Departamento: Ingeniería y Ciencia de los Materiales
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)