Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Host-dependent expression of Rhizobium leguminosarum bv. viciae hydrogenase is controlled at transcriptional and post-transcriptional levels in legume nodules.
Year:2008
Research Areas
  • Microbiology
Information
Abstract
The legume host affects the expression of Rhizobium leguminosarum hydrogenase activity in root nodules. High levels of symbiotic hydrogenase activity were detected in R. leguminosarum bacteroids from different hosts, with the exception of lentil (Lens culinaris). Transcription analysis showed that the NifA-regulated R. leguminosarum hydrogenase structural gene promoter (P1) is poorly induced in lentil root nodules. Replacement of the P1 promoter by the FnrN-dependent promoter of the fixN gene restored transcription of hup genes in lentil bacteroids, but not hydrogenase activity. In the PfixN-hupSL strain, additional copies of the hup gene cluster and nickel supplementation to lentil plants increased bacteroid hydrogenase activity. However, the level of activity in lentil still was significantly lower than in pea bacteroids, indicating that an additional factor is impairing hydrogenase expression inside lentil nodules. Immunological analysis revealed that lentil bacteroids contain reduced levels of both hydrogenase structural subunit HupL and nickel-binding protein HypB. Altogether, results indicate that hydrogenase expression is affected by the legume host at the level of both transcription of hydrogenase structural genes and biosynthesis or stability of nickelrelated proteins HypB and HupL, and suggest the existence of a plant-dependent mechanism that affects hydrogenase activity during the symbiosis by limiting nickel availability to the bacteroid.
International
Si
JCR
Si
Title
MOLECULAR PLANT-MICROBE INTERACTIONS
ISBN
0894-0282
Impact factor JCR
4,275
Impact info
Volume
21
10.1094/MPMI -21-5-0597
Journal number
5
From page
597
To page
604
Month
ENERO
Ranking
Participants
  • Participante: Annita Toffanin (Universidad de Pisa)
  • Autor: Maria Belen Brito Lopez (UPM)
  • Autor: Tomas-Andres Ruiz Argueso (UPM)
  • Autor: Jose Manuel Palacios Alberti (UPM)
  • Autor: Juan Imperial Ródenas (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Asociaciones simbióticas planta-microorganismo
  • Departamento: Biotecnología
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
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