Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Novel arrangement of enhancer sequences for NifA-dependent activation of the hydrogenase gene promoter in Rhizobium leguminosarum bv. viciae
Year:2008
Research Areas
  • Microbiology
Information
Abstract
The transcriptional activation of the NifA-dependent promoter of the Rhizobium leguminosarum hydrogenase structural genes hupSL (P1) has been studied through gel retardation analysis and detailed mutagenesis. Gel retardation analysis indicated the existence of a physical interaction between NifA and the promoter. Extensive mutagenesis followed by in vivo expression analysis showed that three sequences of 4 bases each (􏱏170 ACAA 􏱏167, 􏱏161 ACAA 􏱏158, and 􏱏145 TTGT 􏱏142) are required for maximal stimulation of in vivo transcription of the P1 promoter. The arrangement of these upstream activating sequences (ACAA N5 ACAA N12 TTGT) differs from the canonical 5􏱐ACA N10 TGT 3􏱐 UAS structure involved in NifA-dependent activation of nif/fix genes. Mutant promoter analysis indicated that the relative contribution of each of these sequences to P1 promoter activity increases with its proximity to the transcription start site. Analysis of double mutants altered in two out of the three enhancer sequences suggests that each of these sequences functions in NifA-dependent activation of the P1 promoter in an independent but cooperative mode. The similarities and differences between cis elements of hup and nif/fix promoters suggest that the structure of the P1 promoter has adapted to activation by NifA in order to coexpress hydrogenase and nitrogenase activities in legume nodules.
International
Si
JCR
Si
Title
JOURNAL OF BACTERIOLOGY
ISBN
0021-9193
Impact factor JCR
4,013
Impact info
Volume
190
10.1128/JB.00107-08
Journal number
9
From page
3185
To page
3191
Month
MAYO
Ranking
Participants
  • Participante: Marta Martínez (Universidad Autónoma de Madrid)
  • Autor: Jose Manuel Palacios Alberti (UPM)
  • Autor: Juan Imperial Ródenas (UPM)
  • Participante: Maria Victoria Colombo
  • Autor: Tomas-Andres Ruiz Argueso (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Asociaciones simbióticas planta-microorganismo
  • Departamento: Biotecnología
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
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