Memorias de investigación
Artículos en revistas:
Novel arrangement of enhancer sequences for NifA-dependent activation of the hydrogenase gene promoter in Rhizobium leguminosarum bv. viciae
Año:2008
Áreas de investigación
  • Microbiología
Datos
Descripción
The transcriptional activation of the NifA-dependent promoter of the Rhizobium leguminosarum hydrogenase structural genes hupSL (P1) has been studied through gel retardation analysis and detailed mutagenesis. Gel retardation analysis indicated the existence of a physical interaction between NifA and the promoter. Extensive mutagenesis followed by in vivo expression analysis showed that three sequences of 4 bases each (􏱏170 ACAA 􏱏167, 􏱏161 ACAA 􏱏158, and 􏱏145 TTGT 􏱏142) are required for maximal stimulation of in vivo transcription of the P1 promoter. The arrangement of these upstream activating sequences (ACAA N5 ACAA N12 TTGT) differs from the canonical 5􏱐ACA N10 TGT 3􏱐 UAS structure involved in NifA-dependent activation of nif/fix genes. Mutant promoter analysis indicated that the relative contribution of each of these sequences to P1 promoter activity increases with its proximity to the transcription start site. Analysis of double mutants altered in two out of the three enhancer sequences suggests that each of these sequences functions in NifA-dependent activation of the P1 promoter in an independent but cooperative mode. The similarities and differences between cis elements of hup and nif/fix promoters suggest that the structure of the P1 promoter has adapted to activation by NifA in order to coexpress hydrogenase and nitrogenase activities in legume nodules.
Internacional
Si
JCR del ISI
Si
Título de la revista
JOURNAL OF BACTERIOLOGY
ISSN
0021-9193
Factor de impacto JCR
4,013
Información de impacto
Volumen
190
DOI
10.1128/JB.00107-08
Número de revista
9
Desde la página
3185
Hasta la página
3191
Mes
MAYO
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Participantes
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Asociaciones simbióticas planta-microorganismo
  • Departamento: Biotecnología
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP