Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Characterization by immunocytochemistry of ionic channels in Helix aspersa suboesophageal brain ganglia neurons
Year:2008
Research Areas
  • Medicine,
  • Molecular biology
Information
Abstract
The aim of this work was to characterize several ionic channels in nervous cells of the suboesophageal visceral, left and right parietal, and left and right pleural brain ganglia complex of the snail Helix aspersa by immunocytochemistry. We have studied the immunostaining reaction for a wide panel of eleven polyclonal antibodies raised against mammal antigens as follows: voltage-gated-Na+ channel; voltage-gated-delayed-rectifier-K+ channel; SK2-small-conductance-Ca2+-dependent-K+ channel apamin sensitive; SK3 potassium channel; charybdotoxin-sensitive voltage-dependent potassium channel; BKCa-maxi-conductance-Ca2+-dependent-K+ channel; hyperpolarizationactivated cyclic nucleotide-gated potassium channel 4; G-protein-activated inwardly rectifying potassium channel GIRK2 and voltage-gated-calcium of L, N and P/Q type channels. Our results show positive reaction in neurons, but neither in glia cells nor in processes in the Helix suboesophageal ganglia. Our results suggest the occurrence of molecules in Helix neurons sharing antigenic determinants with mammal ionic channels. The reaction density and distribution of immunoreactive staining within neurons is specific for each one of the antisera tested. The studies of co-localization of immunoreaction, on alternate serial sections of the anterior right parietal ganglion, have shown for several recognized mapped neurons that they can simultaneously be expressed among two and seven different ionic protein channels. These results are considered a key structural support for the interpretation of Helix aspersa neuron electrophysiological activity
International
Si
JCR
Si
Title
HISTOLOGY AND HISTOPATHOLOGY
ISBN
0213-3911
Impact factor JCR
2,007
Impact info
Volume
23
Journal number
0
From page
397
To page
406
Month
ENERO
Ranking
Participants
  • Autor: Ceferino Maestu Unturbe (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Grupo de Bioingeniería y Telemedicina
  • Departamento: Tecnología Fotónica
  • Centro o Instituto I+D+i: Centro de tecnología Biomédica CTB
S2i 2021 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)