Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Brenneria quercina and Serratia spp. isolated from Spanish oak trees: molecular characterization and PCR development
Year:2008
Research Areas
  • Molecular biology
Information
Abstract
During last years of the 20th century, oak decline has been reported in Spain due to several pathogens and/or abiotic factors. One of the causal agents of such decline has been identified as the bacterial phytopathogen Brenneria quercina. To investigate the bacterial variability of this pathogen from different Spanish oak forests, a collection of bacterial isolates from several geographic locations and from different oak species was analysed by sequencing 16S rDNA and using rep-PCR fingerprinting. In this study, 16S rDNA analysis revealed that most of the isolates were Brenneria. A few belonged to the genus Serratia, suggesting a possible involvement of this bacterium in oak disease. All Spanish isolates of B. quercina were grouped by rep-PCR in a homogenous cluster that differed significantly from the B. quercina reference strains from California. The information obtained by the rep-PCR fingerprint analysis was used to develop PCR-primers for the sensitive and specific detection of B. quercina from infected plant tissues.
International
Si
JCR
Si
Title
PLANT PATHOLOGY
ISBN
0032-0862
Impact factor JCR
2,012
Impact info
Volume
57
Journal number
0
From page
812
To page
819
Month
ENERO
Ranking
Participants
  • Autor: Pablo Rodriguez Palenzuela (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Interacciones Moleculares Planta-Patógeno
  • Departamento: Biotecnología
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)