Observatorio de I+D+i UPM

Memorias de investigación
Otras publicaciones:
Flexible Fitting of Protein Models into 3D-EM Maps by Using an Image Registration Algorithm
Áreas de investigación
  • Industria electrónica
In this work we propose an image registration algorithm to automatically fit protein atomic domain models into medium-resolution three-dimensional electron microscopy reconstructions (3D-EM map). The approach employs an image flexible registration algorithm whose optimizer controls the generation of stereo-chemically correct models from a given reference domain belonging to a super-family of proteins. Models are generated by studying the evolutionary structural variability that exists among the domains of the super-family. The proposed algorithm generates models automatically in the correct direction until the optimizer converges to the best fitted one. A local gradient optimizer is employed for this task. This optimization method seems to improve the traditional use of a grid of sampled models, since the model that best fit in the 3D-EM map could not be necessarily included in the grid. Mutual Information similarity measure is used as an alternative to Cross Correlation Coefficient that is traditionally the most widely used in the context of molecular image registration. An additional rigid registration that uses a local gradient optimization is applied between each generated model and the 3D-EM map in order to compensate small misalignments and offsets between initial images.
Lake Tahoe (CA, USA)
4th International Conference on Structural Analysis of Supramolecular Assemblies by Hybrid Methods
Tipo de publicación
Acta de Congreso Internacional
Esta actividad pertenece a memorias de investigación
  • Participante: Carlos O.S. Sorzano (CNB - CSIC)
  • Participante: Julián Atienza-Herrero (CNB - CSIC)
  • Autor: Andres de Santos Lleo (UPM)
  • Participante: Laura Fernández-de-Manuel (UPM)
  • Autor: Maria Jesus Ledesma Carbayo (UPM)
  • Participante: José María Carazo (CNB - CSIC)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Tecnología de imágenes biomédicas
  • Centro o Instituto I+D+i: Centro de tecnología Biomédica CTB
  • Departamento: Ingeniería Electrónica
S2i 2022 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)