Memorias de investigación
Artículos en revistas:
Adaptation of aphid stylectomy for analyses of proteins and mRNAs in barley phloem sap
Año:2008
Áreas de investigación
  • Biología molecular
Datos
Descripción
Sieve tubes are transport conduits not only for photoassimilates but also for macromolecules and other compounds that are involved in sieve tube maintenance and systemic signalling. In order to gain sufficient amounts of pure phloem exudates from barley plants for analyses of the protein and mRNA composition, a previously described stylectomy set-up was optimized. Aphids were placed in sealed cages, which, immediately after microcauterization of the stylets, were flooded with water-saturated silicon oil. The exuding phloem sap was collected with a capillary connected to a pump. Using up to 30 plants and 600 aphids (Rhopalosiphum padi) in parallel, an average of 10 ml of phloem sap could be obtained within 6 h of sampling. In first analyses of the macromolecular content, eight so far unknown phloem mRNAs were identified by cDNA-amplified fragment length polymorphism. Transcripts in barley phloem exudates are related to metabolism, signalling, and pathogen defence, for example coding for a protein kinase and a pathogen- and insect-responsive WIR1A (wheatinduced resistance 1A)-like protein. Further, onedimensional gel electrophoresis and subsequent partial sequencing by mass spectrometry led to the identification of seven major proteins with putative functions in stress responses and transport of mRNAs, proteins, and sugars. Two of the discovered proteins probably represent isoforms of a new phloemmobile sucrose transporter. Notably, two-dimensional electrophoresis confirmed that there are >250 phloem proteins awaiting identification in future studies
Internacional
Si
JCR del ISI
Si
Título de la revista
JOURNAL OF EXPERIMENTAL BOTANY
ISSN
0022-0957
Factor de impacto JCR
3,917
Información de impacto
Volumen
59
DOI
Número de revista
0
Desde la página
3297
Hasta la página
3306
Mes
ENERO
Ranking
Esta actividad pertenece a memorias de investigación
Participantes
  • Participante: A.J.E van Bel
  • Participante: S Deshmukh
  • Participante: Knauer T
  • Autor: Julia Kehr . UPM
  • Participante: F Gaupels,
  • Participante: F Waller
  • Participante: K.-H Kogel,
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP