Memorias de investigación
Artículos en revistas:
Identification of high levels of phytochelatins, glutathione and cadmium in the phloem sap of Brassica napus and role for thiol-peptides in long-distance transport of cadmium
Año:2008
Áreas de investigación
  • Biología molecular
Datos
Descripción
Phytochelatins (PCs) are glutathione-derived peptides that function in heavy metal detoxification in plants and certain fungi. Recent research in Arabidopsis has shown that PCs undergo long-distance transport between roots and shoots. However, it remains unknown which tissues or vascular systems, xylem or phloem, mediate PC translocation and whether PC transport contributes to physiologically relevant long-distance transport of cadmium (Cd) between shoots and roots. To address these questions, xylem and phloem sap were obtained from Brassica napus to quantitatively analyze which thiol species are present in response to Cd exposure. High levels of PCs were identified in the phloem sap within 24 h of Cd exposure using combined mass spectrometry and fluorescence HPLC analyses. Unexpectedly, the concentration of Cd was more than four-fold higher in phloem sap compared to xylem sap. Cadmium exposure dramatically decreased iron levels in xylem and phloem sap whereas other essential heavy metals such as zinc and manganese remained unchanged. Data suggest that Cd inhibits vascular loading of iron but not nicotianamine. The high ratios [PCs]/[Cd] and [glutathione]/[Cd] in the phloem sap suggest that PCs and glutathione (GSH) can function as long-distance carriers of Cd. In contrast, only traces of PCs were detected in xylem sap. Our results suggest that, in addition to directional xylem Cd transport, the phloem is a major vascular system for long-distance source to sink transport of Cd as PC¿Cd and glutathione¿Cd complexes.
Internacional
Si
JCR del ISI
Si
Título de la revista
PLANT JOURNAL
ISSN
0960-7412
Factor de impacto JCR
6,751
Información de impacto
Volumen
54
DOI
Número de revista
0
Desde la página
249
Hasta la página
259
Mes
ENERO
Ranking
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Participantes
  • Participante: D Mendoza-Cózatl
  • Participante: JL Schroeder,
  • Participante: J Torpey
  • Autor: Julia Kehr . UPM
  • Participante: E Butko
  • Participante: E Komives,
  • Participante: F Springer
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP