Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Influence of different reproductive rhythms on serum estradiol and testosterone levels, features of follicular population and atresia rate, and oocyte maturation in controlled suckling rabbits.
Year:2009
Research Areas
  • Animal production
Information
Abstract
The aim of the current work was to analyze the features of ovarian follicular population and their quality in New Zealand white rabbit does synchronized by 24-h controlled doe-litter separation before artificial insemination (AI) during all their reproductive cycles. Synchronized animals were allocated systematically in two groups. A total of 73 rabbit does (group A) were submitted to a 35-day intensive rhythm (AI on day 4 post-partum [pp] and weaning at 25 days of lactation), and 108 rabbit does (group B) were submitted to a 42-day semi-intensive rhythm (AI on day 11 pp and weaning at 35 days of lactation) during 9 months. At the mid-end of their reproductive life, a total of 26 does (5.4 parturitions), under intensive (n = 15) or semi-intensive rhythm (n=11) were either treated in each group with 25 IU eCG 48 h before laparotomy to recover their ovaries (n = 7 for group A and n=6 for group B) (according to the Bioethics Committee of the University) or not synchronized with the hormonal treatment (n=8 for group A and n = 5 for group B). Blood samples were collected at the moment of ovary recovery; morphometrical parameters, number of total follicles and number of follicles ¿1mm in size in the ovarian surface were recorded. Oocytes from follicles of one ovary were recovered and matured in TCM 199 supplemented with 10 ng/ml EGF, 100 ng/ml IGF-I and 10% FCS. The counterpart ovaries were fixed in paraformaldehyde solution for histological studies. Detection of cell apoptosis was determined using the terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labelling (TUNEL) technique. Reproductive performance was affected by the rhythm used, with lower reproductive parameters in the intensive group. The average ovary height and width, the mean number of ¿1mm follicles and the number of total follicles were similar between groups. Serum concentrations of estradiol (E2) and testosterone (T) were significantly lower in group A vs. B (E2 : 232.4±56.1 vs. 399.7±53.0 pg/ml; P < 0.05 and T: 1.07±0.10 vs. 1.68±0.23 ng/ml; P < 0.05). No significant differences were found in follicular population or in the mean follicular apoptosis index between groups. Metaphase II rate was significantly lower in group A vs. B (48.5±3.3 vs. 67.6±3.7%; P < 0.01), as well as the migration rate of cortical granules (12.7±2.7 vs. 38.2±6.6%; P < 0.001). On the other hand, neither follicular population, nuclear maturation rate nor apoptosis ratewere affected by the eCG treatment, but cytoplasmic maturation was higher in animals treated with eCG in group A (29.2% vs. 5.5%; P < 0.01). In conclusion, rabbit does under transient litter separation during their reproductive life have both their serum estradiol and testosterone concentrations and oocyte quality influenced by the intensive rhythm, leading to a decrease in reproductive parameters. Also, both intensive and semi-intensive rhythms seem to be less receptive to eCG treatment than expected.
International
Si
JCR
Si
Title
ANIMAL REPRODUCTION SCIENCE
ISBN
0378-4320
Impact factor JCR
1,89
Impact info
Volume
114
Journal number
4
From page
423
To page
433
Month
ENERO
Ranking
Participants
  • Autor: Maria del Pilar Garcia Rebollar (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Producción Animal
  • Departamento: Producción Animal
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)