Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Multiplicity of infection of a plant virus varies during colonization of its eukaryotic host
Year:2009
Research Areas
  • Crop production
Information
Abstract
The multiplicity of infection (MOI), i.e., the number of virus genomes that infect a cell, is a key parameter in virus evolution, as it determines processes such as genetic exchange among genomes, selection intensity on viral genes, epistatic interactions, and the evolution of multipartite viruses. In fact, the MOI level is equivalent to the virus ploidy during genome expression. Nevertheless, there are few experimental estimates of MOI, particularly for viruses with eukaryotic hosts. Here we estimate the MOI of Tobacco mosaic virus (TMV) in its systemic host, Nicotiana benthamiana. The progress of infection of two TMV genotypes, differently tagged with the green or red fluorescent proteins GFP and RFP, was monitored by determining the number of leaf cell protoplasts that showed GFP, RFP, or GFP and RFP fluorescence at different times postinoculation. This approach allowed the quantitative analysis of the kinetics of infection and estimation of the generation time and the number of infection cycles required for leaf colonization. MOI levels were estimated from the frequencyof cells infected by only TMV-GFP or TMV-RFP. The MOI was high, but it changed during the infection process, decreasing from an initial level of about 6 to a final one of 1 to 2, with most infection cycles occurring at the higher MOI levels. The decreasing MOI can be explained by mechanisms limiting superinfection and/or by genotype competition within double-infected cells, which was shown to occur in coinfected tobacco protoplasts. To our knowledge, this is the first estimate of MOI during virus colonization of a eukaryotic host.
International
Si
JCR
Si
Title
JOURNAL OF VIROLOGY
ISBN
0022-538X
Impact factor JCR
5,308
Impact info
Volume
83
Journal number
0
From page
7487
To page
7494
Month
ENERO
Ranking
Participants
  • Participante: TOMÁS CANTO (CIB-CSIC)
  • Autor: Pablo Gonzalez Jara (UPM)
  • Autor: Fernando Garcia-Arenal Rodriguez (UPM)
  • Autor: Aurora Fraile Perez (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Patología Vegetal
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología
S2i 2019 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
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