Observatorio de I+D+i UPM

Memorias de investigación
Artículos en revistas:
Generation and properties of a new human ventral mesencephalic neural stem cell line
Año:2009
Áreas de investigación
  • Biología celular,
  • Procesado y análisis de la señal
Datos
Descripción
Neural stem cells (NSCs) are powerful research tools for the design and discovery of newapproaches to cell therapy in neurodegenerative diseases like Parkinson's disease. Several epigenetic and genetic strategies have been tested for long-term maintenance and expansion of these cells in vitro. Here we report the generation of a new stable cell line of human neural stem cells derived from ventral mesencephalon (hVM1) based on v-myc immortalization. The cells expressed neural stem cell and radial glia markers like nestin, vimentin and 3CB2 under proliferation conditions. After withdrawal of growth factors, proliferation and expression of v-myc were dramatically reduced and the cells differentiated into astrocytes, oligodendrocytes and neurons. hVM1 cells yield a large numberof dopaminergic neurons (about 12% of total cells are TH+) after differentiation, which also produce dopamine. In addition to proneural genes (NGN2, MASH1), differentiated cells show expression of several genuine mesencephalic dopaminergic markers such as: LMX1A, LMX1B, GIRK2, ADH2, NURR1, PITX3, VMAT2 and DAT, indicating that they retain their regional identity. Our data indicate that this cell line and its clonal derivatives may constitute good candidates for the study of development and physiology of human dopaminergic neurons in vitro, and to develop tools for Parkinson's disease cell replacement preclinical research and drug testing.
Internacional
Si
JCR del ISI
No
Título de la revista
ELSEIVER
ISSN
00000000
Factor de impacto JCR
0
Información de impacto
Volumen
DOI
10.1016/j.yexcr.2009.03.011
Número de revista
0
Desde la página
2
Hasta la página
15
Mes
ENERO
Ranking
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Milagros Ramos Gomez (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Grupo de Bioingeniería y Telemedicina
  • Centro o Instituto I+D+i: Centro de tecnología Biomédica CTB
  • Departamento: Tecnología Fotónica
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