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Descripción
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| We present a simple and parameter-free nuclei tracking method for reconstructing cell dynamics in fluorescence 3D+t images of embryogenesis. The strategy is based on the use of the mathematical morphology operators directly in the 4D image. The morphological reconstruction of a marker - manually or automatically selected- in an initial spatio-temporal position generates a connected path over the time representing the cell migration. Thus, the processing provides a coherent spatio-temporal estimation of cell movement. The algorithm has been validated on in vivo images of early zebrafish and sea urchin embryogenesis acquired with two-photon laser scanning microscopy providing mean tracking rates above 98% per time step. | |
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Internacional
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Si |
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Nombre congreso
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31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 2009) |
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Tipo de participación
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960 |
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Lugar del congreso
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Minneapolis MN USA |
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Revisores
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Si |
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ISBN o ISSN
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1557-170X |
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DOI
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Fecha inicio congreso
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02/09/2009 |
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Fecha fin congreso
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06/09/2009 |
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Desde la página
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970 |
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Hasta la página
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973 |
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Título de las actas
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Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 2009) |