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Descripción
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| Plants defend themselves from nematode using a variety of mechanisms including localized necrosis at the site of infection (the hypersensitive response, HR), increased expression of defence-related proteins, production of antimicrobial compounds, lignin formation, and oxidative burst. Plant class III peroxidases (PRXs) have been proposed as key regulators of the level of extracellular H2O2 and as producers of the extremely reactive oxygen species (ROS; Bolwell et al. 2002; Bindschedler et al. 2006), depending on whether the peroxidative (catalytic) or the hydroxylic cycles of the enzyme are operating (Kawano 2003; Passardi et al. 2004). Current models of plant defence responses include peroxidase-generated radicals and ROS as catalysts of covalent cell-wall modifications (Ros Barcelo 1995; Østergaard et al. 2000) as well as signals for cell-death reactions (Passardi et al. 2004). Involvement of peroxidases in wheat defence was suggested by selectively induced expression of the pox2 peroxidase gene in leaves of hexaploid wheat (Båga et al. 1995) as well as other peroxidase genes in diploid wheat, Triticum monococcum, when challenged by powdery mildew fungus (Liu et al. 2005). In addition, hydrogen peroxide production is a known response of host plants to root-knot (Melillo et al. 2006; Das et al. 2008) and cyst nematode invasion (Waetzing et al. 1999). In previous studies, findings obtained by our group (Andrés et al. 2001; Montes et al. 2003 and 2004) demonstrated that cyst nematode infection enhances cationic and anionic PRX isoforms activity in wheat roots carrying Cre2, Cre5 or Cre7 resistance genes. The objective of the present study was to asses the involvement of peroxidases in the defence response and by which mechanism. We carried out a functional characterization of these isoforms studying their expression and localization in resistant (H-93-8) and susceptible (H-10-15) wheat lines challenged by the cereal cyst nematode (CCN) Heterodera avenae. The data presented here suggest that a number of PRX transcripts might play a role in the constitutive and inducible defence mechanisms, as reported in other host-pathogen interactions (Liu et al. 2005; Schweizer 2008). | |
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Internacional
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Si |
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Nombre congreso
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Cost 872 Workshop & Meeting |
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Tipo de participación
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960 |
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Lugar del congreso
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Toledo, España |
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Revisores
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Si |
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ISBN o ISSN
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DOI
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Fecha inicio congreso
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09/07/2009 |
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Fecha fin congreso
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11/07/2009 |
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Título de las actas
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