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Memorias de investigación
Communications at congresses:
Cryopreservation tolerance of seeds of the endangered species Tuberaria major
Year:2009
Research Areas
  • Botany
Information
Abstract
In this work we studied the cryopreservation tolerance of Tuberaria major (Cistaceae) seeds, an endangered plant species endemic from the Algarve region (South of Portugal). Seeds were transferred to 2 ml polypropylene cryovials and directly plunged in liquid nitrogen (LN) for 1 month. Seeds were removed from storage and allowed to warm at room temperature for few hours and then surface sterilized, heated at 100ºC during 60 min and sown; a group of seeds were sown without heat treatment. Low germination percentages were attained from cryopreserved seeds that were not submitted to any pre-treatment before sowing. This percentage increased to values similar to those of non-cryopreserved seeds when the heat treatment was applied. These results demonstrate that cryopreservation did not affect the germination capacity but, a heat shock is also essential after cryopreservation to enhance the final germination percentage. In conclusion, it was observed that heat stimulated germination of T. major seeds and cryopreservation could provide a method for long-term storage of germplasm from this endangered species, since no decrease on germination percentage was observed after cryopreservation and the embryos grew into normal seedlings without morphological abnormalities.
International
Si
Congress
1st International Symposium on Cryopreservation in Horticultural Species
960
Place
Lovaina, Bélgica
Reviewers
Si
ISBN/ISSN
Start Date
05/04/2009
End Date
08/04/2009
From page
0
To page
0
Acta Horticulturae. En prensa.
Participants
  • Autor: FERNANDES L.
  • Autor: ROMAO A.
  • Autor: Felix Perez Garcia (UPM)
  • Autor: S. GONÇALVES
  • Autor: M. Elena Gonzalez Benito (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Biodiversidad y conservación de recursos fitogenéticos
  • Departamento: Biología Vegetal
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