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Descripción
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| In this work we studied the cryopreservation tolerance of Tuberaria major (Cistaceae) seeds, an endangered plant species endemic from the Algarve region (South of Portugal). Seeds were transferred to 2 ml polypropylene cryovials and directly plunged in liquid nitrogen (LN) for 1 month. Seeds were removed from storage and allowed to warm at room temperature for few hours and then surface sterilized, heated at 100ºC during 60 min and sown; a group of seeds were sown without heat treatment. Low germination percentages were attained from cryopreserved seeds that were not submitted to any pre-treatment before sowing. This percentage increased to values similar to those of non-cryopreserved seeds when the heat treatment was applied. These results demonstrate that cryopreservation did not affect the germination capacity but, a heat shock is also essential after cryopreservation to enhance the final germination percentage. In conclusion, it was observed that heat stimulated germination of T. major seeds and cryopreservation could provide a method for long-term storage of germplasm from this endangered species, since no decrease on germination percentage was observed after cryopreservation and the embryos grew into normal seedlings without morphological abnormalities. | |
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Internacional
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Si |
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Nombre congreso
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1st International Symposium on Cryopreservation in Horticultural Species |
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Tipo de participación
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960 |
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Lugar del congreso
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Lovaina, Bélgica |
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Revisores
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Si |
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ISBN o ISSN
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DOI
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Fecha inicio congreso
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05/04/2009 |
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Fecha fin congreso
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08/04/2009 |
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Desde la página
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0 |
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Hasta la página
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0 |
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Título de las actas
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Acta Horticulturae. En prensa. |